Description

Butanoic Acid, 4-(2-Pyridinyldithio)-2-Sulfo-, 1-(2,5-Dioxo-1-Pyrrolidinyl) Ester is a specialized heterobifunctional crosslinking reagent designed for advanced bioconjugation and protein modification workflows. Its core value lies in the precise, controlled coupling of biomolecules, offering researchers a reliable tool for constructing stable, site-specific conjugates. This compound is essential for professionals in biopharmaceutical R&D, diagnostic assay development, and advanced materials science who require high-purity, functional linkers for creating next-generation therapeutic and diagnostic platforms.

Application

• Bioconjugation and Protein-Protein Crosslinking: For creating stable antibody-drug conjugates (ADCs), enzyme complexes, and other therapeutic protein constructs.
• Surface Immobilization Chemistry: Functionalizing gold surfaces, nanoparticles, or biosensor chips via the pyridyldithiol group for SPR, ELISA, or other diagnostic platforms.
• Polymer and Biomaterial Modification: Introducing sulfonate and reactive ester groups into hydrogels, polymers, or drug delivery carriers to enhance functionality and biocompatibility.
• Peptide and Small Molecule Labeling: Attaching fluorescent dyes, affinity tags (e.g., biotin), or other probes to peptides or small molecules via amine-reactive chemistry.
• Affinity Chromatography Ligand Coupling: Covalently immobilizing proteins or peptides onto solid supports for purification or capture applications.
• Stable Disulfide Bond Formation: Used in reversible crosslinking strategies for studying protein dynamics or creating stimuli-responsive delivery systems.

Basic Information

Product Name	Butanoic Acid, 4-(2-Pyridinyldithio)-2-Sulfo-, 1-(2,5-Dioxo-1-Pyrrolidinyl) Ester
CAS No.	1193111-39-5
Molecular Formula	C14H13N3O7S3
Molecular Weight	431.46 g/mol
Synonyms	4-(2-Pyridinyldithio)-2-sulfobutanoic acid 1-(2,5-dioxo-1-pyrrolidinyl) ester; 2-Sulfo-4-(2-pyridinyldithio)butanoic acid N-hydroxysuccinimide ester; Sulfo-SPDP; SPDP sulfonate; NHS ester of 4-(2-pyridinyldithio)-2-sulfobutanoic acid; Heterobifunctional crosslinker SPDP-sulfonate; 2-Sulfosuccinimidyl 4-(2-pyridinyldithio)butanoate; Reactive ester pyridyldithiol crosslinker
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical reagent adheres to stringent quality protocols. Every batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and functional integrity. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing assay results and impurity profiles. Our quality system is designed to support GMP-compatible and research-grade applications, ensuring reliability and consistency for your critical experiments and process development.

Storage

Preserve in a tightly closed container, protected from light. This product is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). For long-term stability, store desiccated at -20°C. Under these conditions, the product is typically stable for 24 months. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, water (partial)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.