Description

Uv Cleavable Biotin-Peg2-Azide is a specialized heterobifunctional crosslinker designed for controlled bioconjugation and affinity purification workflows. Its core value lies in the integration of a biotin handle for streptavidin capture, a photocleavable linker for gentle, light-triggered elution, and an azide group for bioorthogonal Click Chemistry. This trifunctional design is essential for researchers and developers in proteomics, drug discovery, and diagnostic assay development who require precise, reversible isolation of biomolecules.

Application

• Affinity Purification & Pull-Down Assays: Immobilize biotinylated targets on streptavidin beads, followed by gentle UV light elution to recover native, unmodified biomolecules.
• Proteomic Sample Preparation: Isolate and enrich specific protein complexes or post-translationally modified proteins for downstream mass spectrometry analysis.
• Controlled Surface Functionalization: Create reversibly biotinylated surfaces or nanoparticles for sequential binding and release applications in biosensor development.
• Activity-Based Protein Profiling (ABPP): Conjugate via Click Chemistry to activity-based probes for the isolation and identification of active enzymes.
• Diagnostic Reagent Development: Used in the construction of diagnostic assays requiring a clean capture-and-release mechanism to reduce background and improve signal-to-noise ratios.
• Antibody-Drug Conjugate (ADC) Research: Facilitate the study of linker stability and payload release mechanisms in model conjugation systems.

Basic Information

Product Name	Uv Cleavable Biotin-Peg2-Azide
CAS No.	1192802-98-4
Molecular Formula	C₂₄H₄₁N₇O₇S
Molecular Weight	571.70 g/mol
Synonyms	Biotin-PEG2-Azide, PC Biotin-PEG2-N3; Photocleavable Biotin Azide; 2-[2-[2-[2-[5-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethyl (2-azidoethyl)carbamate; Biotinylated Photocleavable Linker; UV-sensitive Biotin-Azide Crosslinker
EINECS	Contact for details

Quality Control

Every batch of Uv Cleavable Biotin-Peg2-Azide is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. We provide a detailed Certificate of Analysis (COA) with each shipment, confirming identity, purity, and functional integrity through techniques such as HPLC, NMR, and mass spectrometry. Our quality commitment ensures the reagent performs reliably in sensitive downstream applications.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Functional Test (Click Reaction)	≥90% conjugation efficiency
UV Cleavage Efficiency	≥85% release upon irradiation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.