Description

17β-Estradiol-16,16,17-D3 3-Benzoate is a high-purity, deuterium-labeled internal standard critical for ensuring analytical accuracy in quantitative bioanalysis. This compound is specifically designed for use in Liquid Chromatography-Mass Spectrometry (LC-MS/MS) applications, providing unmatched reliability for trace-level quantification. It is an essential tool for pharmaceutical R&D, clinical research, and forensic toxicology laboratories requiring precise measurement of estradiol and its metabolites.

Application

• Internal Standard for LC-MS/MS: Primary application as a stable isotope-labeled internal standard for the accurate quantification of 17β-estradiol and its derivatives in complex biological matrices.
• Pharmaceutical R&D: Used in pharmacokinetic (PK) and pharmacodynamic (PD) studies, drug metabolism research, and bioequivalence testing for hormone-based therapies.
• Clinical Diagnostics & Research: Enables precise measurement of estrogen levels in serum, plasma, and tissue samples for endocrine disorder studies and hormone monitoring.
• Forensic & Anti-Doping Analysis: Critical for the confirmatory testing of anabolic steroids and endogenous hormones in forensic toxicology and sports doping control.
• Environmental & Food Safety Testing: Supports analytical methods for detecting estrogenic compound residues in environmental water samples and food products.
• Method Development & Validation: Serves as a key reference material for developing, optimizing, and validating robust analytical methods in regulated laboratories.

Basic Information

Product Name	17β-Estradiol-16,16,17-D3 3-Benzoate
CAS No.	1192354-74-7
Molecular Formula	C25H25D3O3
Molecular Weight	379.51 g/mol
Synonyms	Estradiol-16,16,17-d3 3-benzoate; [16,16,17-2H3]-Estradiol 3-benzoate; Deuterated Estradiol Benzoate; 17β-Estradiol-16,16,17-d3 3-benzoate; E2-d3 Benzoate; 1,3,5(10)-Estratrien-3,17β-diol-16,16,17-d3 3-benzoate; (17β)-3-(Benzoyloxy)-estra-1,3,5(10)-trien-16,16,17-d3-17-ol
EINECS	Contact for details

Quality Control

Every batch of 17β-Estradiol-16,16,17-D3 3-Benzoate is manufactured and tested under strict quality management systems. We guarantee high chemical and isotopic purity to meet the exacting demands of analytical reference standard applications. Comprehensive testing includes identity confirmation, assay (HPLC), and determination of deuterium enrichment. A detailed Certificate of Analysis (COA) is provided with each shipment, ensuring full traceability and compliance with your method validation requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as indicated on the product label or COA. This product is hygroscopic (moisture-sensitive). For long-term storage, consider storing under an inert atmosphere or in a desiccator to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (deuterium enrichment)	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.