Description

Hydrochlorothiazide-13C,D2 is a stable isotope-labeled analog of the widely prescribed thiazide diuretic, Hydrochlorothiazide, specifically designed for use as an internal standard in quantitative analytical methods. This high-purity compound is essential for ensuring accuracy and reliability in mass spectrometry-based analyses, such as LC-MS/MS, by correcting for variations in sample preparation and instrument response. It is a critical material for pharmaceutical research and development, clinical laboratories, and contract research organizations (CROs) engaged in drug metabolism and pharmacokinetics (DMPK) studies, bioequivalence testing, and therapeutic drug monitoring.

Application

• Internal Standard for Quantitative LC-MS/MS Analysis: Primary use as a reference compound in the precise quantification of Hydrochlorothiazide and its metabolites in biological matrices (plasma, serum, urine).
• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Enables accurate tracking of drug absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical research.
• Bioanalytical Method Development and Validation: Serves as a critical component in developing and validating robust, regulatory-compliant analytical methods for bioequivalence and clinical trials.
• Therapeutic Drug Monitoring (TDM): Used in clinical laboratory assays to accurately measure drug concentrations in patient samples for dose optimization.
• Pharmaceutical Impurity Profiling and Stability Studies: Aids in the identification and quantification of degradants or related substances in drug substance and product analysis.
• Forensic and Toxicology Analysis: Supports accurate quantification in forensic investigations involving diuretic agents.

Basic Information

Product Name	Hydrochlorothiazide-13C,D2
CAS No.	1190006-03-1
Molecular Formula	C713CH6D2ClN3O4S2
Molecular Weight	~300.19 g/mol (exact mass varies with isotopic enrichment)
Synonyms	6-Chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide-13C,d2; HCTZ-13C,d2; 3,4-Dihydro-6-chloro-7-sulfamoyl-2H-1,2,4-benzothiadiazine 1,1-dioxide-13C,d2; Isotopically Labeled Hydrochlorothiazide; [13C,d2]-Hydrochlorothiazide; Hydrochlorothiazide (phenyl-13C,d2); Stable Isotope-Labeled HCTZ
EINECS	Contact for details

Quality Control

Every batch of Hydrochlorothiazide-13C,D2 is manufactured and analyzed under strict quality systems to ensure the highest standards of isotopic purity, chemical purity, and stability required for sensitive analytical applications. Our products undergo rigorous quality testing, including NMR and mass spectrometry for isotopic enrichment verification and HPLC for chemical purity assessment. Certificates of Analysis (COA) detailing batch-specific results for assay, isotopic purity, and related substances are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and must be kept in a desiccated environment to maintain stability and purity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to expected mass spectrum
Purity (HPLC)	≥98.0%
Isotopic Purity	≥99 atom % 13C; ≥98 atom % D
Chemical Purity	≥95% (by NMR)
Water Content (KF)	≤1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.