Description

Trimethoprim-13C3 is a stable isotope-labeled analog of the antibiotic Trimethoprim, where three carbon atoms are replaced with the 13C isotope. This compound is essential as an internal standard in quantitative mass spectrometry, providing unmatched accuracy and reliability for analytical method development and validation. It is a critical tool for pharmaceutical researchers, clinical laboratories, and contract research organizations (CROs) engaged in drug metabolism studies, pharmacokinetic analysis, and ensuring regulatory compliance in bioanalytical testing.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a high-purity internal standard for the precise and accurate quantification of Trimethoprim and its metabolites in complex biological matrices.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in tracer studies to investigate the absorption, distribution, metabolism, and excretion (ADME) profile of Trimethoprim.
• Clinical Research & Therapeutic Drug Monitoring (TDM): Enables reliable measurement of Trimethoprim concentrations in patient serum or plasma to optimize dosing regimens and ensure therapeutic efficacy.
• Pharmaceutical Impurity Profiling: Aids in the identification and quantification of process-related impurities or degradation products in active pharmaceutical ingredient (API) batches.
• Veterinary Drug Residue Analysis: Critical for developing compliant methods to detect and quantify Trimethoprim residues in food-producing animals to ensure food safety.
• Environmental Fate Studies: Employed in research to trace the environmental breakdown and persistence of antibiotic compounds.

Basic Information

Product Name	Trimethoprim-13C3
CAS No.	1189970-95-3
Molecular Formula	C1313C3H19N4O3
Molecular Weight	294.30 g/mol (for 13C3 labeled form)
Synonyms	5-[(3,4,5-Trimethoxyphenyl)methyl]-2,4-pyrimidinediamine-13C3; 2,4-Diamino-5-(3,4,5-trimethoxybenzyl)pyrimidine-13C3; Trimethoprim (13C3); [13C3]-Trimethoprim; Trimethoprim-13C3 Internal Standard; Isotopically Labeled Trimethoprim; TMP-13C3
EINECS	Contact for details

Quality Control

Every batch of Trimethoprim-13C3 is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity, supported by comprehensive analytical data including NMR, Mass Spectrometry, and HPLC. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity (typically ≥98% chemical purity, ≥99% isotopic purity), and suitability for use as an internal standard. Our quality commitment ensures compliance with the rigorous demands of analytical and regulatory science.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 99.0 atom % 13C
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.