Description

Chlorothiazide-13C,15N2 is a stable isotope-labeled analog of the thiazide diuretic Chlorothiazide, specifically enriched with carbon-13 and nitrogen-15 at designated positions. This compound is an essential internal standard and reference material for quantitative mass spectrometry, ensuring unparalleled accuracy in pharmacokinetic, metabolic, and bioanalytical studies. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in advanced analytical method development and drug metabolism research for precise quantification.

Application

• Primary use as an Internal Standard (IS) for the accurate quantification of Chlorothiazide in biological matrices (e.g., plasma, urine, tissue) via LC-MS/MS and GC-MS.
• Critical reference material for conducting drug metabolism and pharmacokinetics (DMPK) studies, including absorption, distribution, metabolism, and excretion (ADME) profiling.
• Essential component in the development and validation of bioanalytical methods to meet FDA, EMA, and ICH regulatory guidelines.
• Used in isotope dilution mass spectrometry (IDMS) to achieve high-precision measurements and correct for analyte loss during sample preparation.
• Facilitates research into the mechanism of action and metabolic pathways of thiazide diuretics.
• Supports clinical research and therapeutic drug monitoring (TDM) programs requiring the highest level of analytical certainty.

Basic Information

Item	Detail
Product Name	Chlorothiazide-13C,15N2
CAS No.	1189440-79-6
Molecular Formula	C713CH5ClN152O4S2
Molecular Weight	Contact for details
Synonyms	6-Chloro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide-13C,15N2; 6-Chloro-7-sulfamoyl-1,2,4-benzothiadiazine 1,1-dioxide-13C,15N2; [13C,15N2]-Chlorothiazide; Chlorothiazide (13C,15N2); Isotopically Labeled Chlorothiazide; Chlorothiazide-d0 (13C,15N2); Stable Isotope-Labeled Chlorothiazide
EINECS	Contact for details

Quality Control

Every batch of Chlorothiazide-13C,15N2 is manufactured and analyzed under strict quality systems to ensure exceptional isotopic and chemical purity. Our products undergo rigorous quality testing to ensure compliance with industry standards for stable isotope-labeled compounds. Certificates of Analysis (COA) are available upon request and provide comprehensive data, including assay by HPLC, isotopic enrichment by MS, and identification by spectroscopic methods.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and must be kept under inert conditions or in a desiccator after opening to maintain stability and purity. For long-term storage, consider storing at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Enrichment	≥99 atom % 13C; ≥99 atom % 15N
Chemical Purity	≥95%
Water Content (KF)	≤1.0%
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.