Description

4-Anilinomethylenepentenedioic Acid-D5 5-Methyl Ester is a high-purity, deuterium-labeled derivative of a key pentenedioic acid intermediate. This compound is specifically engineered for use as an internal standard in quantitative mass spectrometry, providing superior accuracy and reliability in analytical workflows. It is essential for researchers and quality control laboratories in the pharmaceutical, agrochemical, and advanced material sectors who require precise isotopic dilution analysis for method development and validation.

Application

• Primary use as a deuterated internal standard (IS) for Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis.
• Critical for quantitative bioanalysis in pharmacokinetic and metabolomic studies within pharmaceutical R&D.
• Enables accurate quantification of its non-deuterated counterpart or related metabolites in complex biological matrices (e.g., plasma, urine, tissue).
• Supports method development and validation for regulatory compliance in drug discovery and development.
• Used in environmental analysis for tracing and quantifying specific chemical residues.
• Applicable in isotope labeling studies to investigate chemical reaction mechanisms and pathways.
• Potential use in the synthesis of labeled compounds for advanced material science research.

Basic Information

Product Name	4-Anilinomethylenepentenedioic Acid-D5 5-Methyl Ester
CAS No.	1185237-80-2
Molecular Formula	C14H10D5NO4
Molecular Weight	Contact for details
Synonyms	4-[(Anilinomethylene)-D5]pent-2-enedioic acid 5-methyl ester; 2-Butenedioic acid, 4-[(phenylamino)methylene-D5]-, 5-methyl ester; Deuterated 4-Anilinomethylenepentenedioic Acid Methyl Ester; 4-Anilinomethylene-2-pentenedioic Acid-D5 Methyl Ester; 4-(Anilinomethylene)glutaconic Acid-D5 Monomethyl Ester; 4-AMPDA-D5 5-ME; Internal Standard for 4-Anilinomethylenepentenedioic Acid Analysis
EINECS	Contact for details

Quality Control

Every batch of our deuterated standards is produced under strict quality control protocols to ensure isotopic purity, chemical purity, and stability. We guarantee high chemical and isotopic purity suitable for sensitive analytical applications. Comprehensive testing includes NMR (1H, 13C), mass spectrometry for isotopic enrichment verification, and HPLC for chemical purity assessment. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting batch-specific data.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as indicated on the label or CoA. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed in a dry environment. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Purity (D-content, MS)	≥98 atom % D
Water Content (KF)	≤0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.