Description

1,3,7-Trimethyluric Acid-13C4，15N3 is a high-purity, multi-isotope labeled internal standard essential for advanced quantitative mass spectrometry. This compound provides unmatched accuracy and reliability in the analysis of caffeine and its metabolites by correcting for matrix effects and instrument variability. It is a critical reagent for pharmaceutical R&D, clinical research, and forensic toxicology laboratories requiring precise and reproducible bioanalytical data.

Application

• Quantitative Bioanalysis: Primary use as an internal standard for the accurate LC-MS/MS quantification of caffeine, theobromine, theophylline, and their metabolites in biological matrices (plasma, serum, urine).
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Enables precise tracking of drug metabolism and clearance rates in clinical trials for xanthine-based pharmaceuticals.
• Forensic & Doping Control: Critical for the definitive identification and quantification of stimulants in forensic toxicology and anti-doping programs.
• Metabolomics Research: Used in stable isotope dilution assays to map purine metabolic pathways and investigate related disorders.
• Method Development & Validation: Serves as a key component in developing and validating GLP/GCP-compliant analytical methods for regulatory submissions.
• Nutraceutical & Food Safety Testing: Ensures accurate measurement of caffeine content and purity in dietary supplements, energy drinks, and food products.

Basic Information

Product Name	1,3,7-Trimethyluric Acid-13C4，15N3
CAS No.	1173022-55-3
Molecular Formula	C13C4H1015N3O3
Molecular Weight	~205.10 (Exact mass varies by isotopic enrichment)
Synonyms	1,3,7-Trimethyluric Acid-13C4,15N3; TMUA-13C4,15N3; [13C4,15N3]-1,3,7-Trimethyluric Acid; 1,3,7-Trimethyl-2,6,8-trioxopurine-13C4,15N3; Isotopically Labeled Caffeine Metabolite Standard; Stable Isotope Labeled Internal Standard (SIL-IS); [13C4,15N3]-TMU
EINECS	Contact for details

Quality Control

Every batch of 1,3,7-Trimethyluric Acid-13C4，15N3 is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity, with comprehensive testing via NMR (1H, 13C), HPLC, and mass spectrometry to confirm structure, enrichment, and absence of unlabeled species. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting batch-specific purity, isotopic enrichment, and chromatographic data to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a cool, dry place. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C; ≥ 99 atom % 15N
Chemical Purity (by 1H NMR)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.