Description

5-Hydroxymebendazole-D3 CAS NO 1173020-86-4 is a deuterium-labeled internal standard of the primary metabolite of the anthelmintic drug mebendazole. This high-purity reference material is critical for ensuring the accuracy and reliability of quantitative bioanalytical methods, particularly in pharmacokinetic and residue depletion studies. It is an essential tool for pharmaceutical R&D laboratories, contract research organizations (CROs), and regulatory testing facilities focused on veterinary and human medicine.

Application

• Quantitative Bioanalysis: Serves as a stable isotope-labeled internal standard for the precise quantification of 5-hydroxymebendazole and its parent compound in biological matrices (e.g., plasma, serum, tissue) using LC-MS/MS.
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Enables accurate measurement of drug metabolite concentrations over time to determine absorption, distribution, metabolism, and excretion (ADME) profiles.
• Veterinary Drug Residue Testing: Used in validated methods to monitor mebendazole and metabolite levels in food-producing animals to ensure compliance with maximum residue limits (MRLs) for food safety.
• Method Development and Validation: A key component in developing, optimizing, and validating robust analytical methods in compliance with FDA, EMA, and other regulatory guidelines.
• Clinical Research: Supports clinical trials involving mebendazole by providing reliable data on metabolite formation and exposure in human subjects.
• Quality Control in Manufacturing: Can be utilized in impurity profiling and stability-indicating assays for mebendazole-based pharmaceutical products.

Basic Information

Product Name	5-Hydroxymebendazole-D3
CAS No.	1173020-86-4
Molecular Formula	C₁₆H₁₀D₃N₃O₃
Molecular Weight	298.31 g/mol
Synonyms	5-Hydroxy Mebendazole D3; 5-(Methoxy-d3-carbonyl)-1H-benzimidazol-2-yl carbamic acid methyl ester; Mebendazole Metabolite D3; 5-OH Mebendazole D3; Deuterated 5-Hydroxymebendazole; [Methoxy-d3]-5-hydroxymebendazole; MBZ Metabolite D3; Internal Standard for 5-Hydroxymebendazole
EINECS	Contact for details

Quality Control

Every batch of 5-Hydroxymebendazole-D3 is manufactured under controlled conditions and undergoes rigorous analytical characterization to ensure identity, purity, and isotopic enrichment. Our quality assurance protocol includes advanced techniques such as NMR (¹H, ¹³C) for structural confirmation, HPLC and LC-MS for purity assessment, and MS for verification of deuterium incorporation. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with each shipment, supporting traceability and compliance with GLP/GMP principles for reference standards.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label or COA. This compound is hygroscopic (moisture-sensitive); the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccant use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Deuterium Incorporation)	≥ 98 atom % D
Chemical Purity	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.