Description

1-Acetyl-2,2,5,5-Tetramethyl-3-Pyrroline-3-Carboxylic Acid, n-Hydroxysuccinimide Ester is a high-purity, bifunctional chemical building block designed for advanced bioconjugation and labeling applications. Its core value lies in its dual functionality, combining a reactive NHS ester for efficient amine coupling with a stable nitroxide radical precursor for spin labeling in Electron Paramagnetic Resonance (EPR) spectroscopy. This compound is essential for researchers and manufacturers in pharmaceutical development, structural biology, and advanced materials science who require reliable, site-specific protein and peptide modification.

Application

• Spin Labeling for EPR/ESR Spectroscopy: Acts as a key precursor for synthesizing site-directed spin labels to study protein dynamics, structure, and conformational changes.
• Bioconjugation & Protein Modification: The NHS ester group enables stable amide bond formation with primary amines on proteins, antibodies, and peptides for labeling and functionalization.
• Proteomics & Biomolecular Research: Used in the development of probes and tools for investigating protein-protein interactions, folding, and localization.
• Pharmaceutical Intermediate: Serves as a critical synthetic intermediate in the development of targeted therapeutics and diagnostic agents.
• Polymer & Materials Science: Incorporated into polymers and surfaces to introduce stable radical sites or specific functional handles for advanced material properties.
• Crosslinking Agent: Can be utilized to create covalent crosslinks between biomolecules for stabilization or complex formation studies.

Basic Information

Item	Details
Product Name	1-Acetyl-2,2,5,5-Tetramethyl-3-Pyrroline-3-Carboxylic Acid, n-Hydroxysuccinimide Ester
CAS No.	1076198-74-7
Molecular Formula	C15H22N2O5
Molecular Weight	310.35 g/mol
Synonyms	1-Acetyl-2,2,5,5-tetramethyl-δ3-pyrroline-3-carboxylic acid N-hydroxysuccinimide ester; ATMP-NHS Ester; 3-Carboxy-2,2,5,5-tetramethylpyrrolin-1-yloxyl, N-hydroxysuccinimide ester, acetylated; 3-Carboxy-PROXYL, NHS ester, acetylated; Nitroxide spin label NHS ester; EPR spin labeling reagent; PROXYL-based NHS active ester
EINECS	Contact for details

Quality Control

Our production of 1-Acetyl-2,2,5,5-Tetramethyl-3-Pyrroline-3-Carboxylic Acid, n-Hydroxysuccinimide Ester adheres to stringent quality protocols to ensure batch-to-batch consistency and high purity for sensitive research applications. All materials are characterized by multiple analytical techniques, and comprehensive Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with each shipment. We support compliance with cGMP and ISO 9001 standards for pharmaceutical intermediate applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to reach room temperature before opening to minimize moisture condensation. For long-term storage, consider storage under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0% (Area %)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.