Description

(L)-2-[(T-Boc)Amino]-2-[(5-Fluoresceinyl)Aminocarbonyl]Ethyl Methanethiosulfonate is a specialized, high-purity biochemical reagent designed for advanced research and development. This compound is critical for site-specific protein and peptide labeling, enabling precise bioconjugation and fluorescence tagging. It is primarily utilized by researchers and manufacturers in the fields of biopharmaceuticals, proteomics, and diagnostic assay development who require reliable, high-quality building blocks for complex molecular constructs.

Application

• Site-Specific Protein Labeling: Used as a thiol-reactive probe for introducing a fluorescent fluorescein tag onto cysteine residues in proteins and peptides.
• Bioconjugation Reagent: A key intermediate in the synthesis of fluorescent antibody-drug conjugates (ADCs) and other targeted therapeutic agents.
• Proteomics Research: Employed in fluorescence-based assays for protein detection, quantification, and cellular imaging studies.
• Diagnostic Kit Development: Serves as a critical component in the manufacturing of fluorescent probes for immunoassays and biosensors.
• Chemical Biology Tool: Facilitates the study of protein structure, function, and interactions through fluorescence resonance energy transfer (FRET) and other techniques.
• Peptide Modification: Enables the controlled attachment of reporter groups to synthetic peptides for pharmacological and biochemical studies.

Basic Information

Product Name	(L)-2-[(T-Boc)Amino]-2-[(5-Fluoresceinyl)Aminocarbonyl]Ethyl Methanethiosulfonate
CAS No.	1042688-20-9
Molecular Formula	C32H32N2O10S2
Molecular Weight	668.73 g/mol
Synonyms	Boc-Lys(FITC)-MTS; Nε-Boc-Nα-(5-fluoresceinyl)-L-lysine Methanethiosulfonate; (S)-2-((tert-Butoxycarbonyl)amino)-6-((5-fluoresceinyl)carboxamido)hexyl methanesulfonothioate; Fluorescein-Boc-Lysine-MTS; Boc-Lys(FITC)-Methanethiosulfonate; Thiol-reactive Fluorescein Labeling Reagent; 5-FITC-Boc-Lys(MTS)
EINECS	Contact for details

Quality Control

Our production of (L)-2-[(T-Boc)Amino]-2-[(5-Fluoresceinyl)Aminocarbonyl]Ethyl Methanethiosulfonate adheres to stringent quality protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided to guarantee the material meets the exacting standards required for bioconjugation and pharmaceutical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Single Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.