Description

Cyanine5 NHS Ester is a near-infrared fluorescent labeling reagent widely used for the covalent conjugation of biomolecules. This compound is critical for creating stable, bright fluorescent probes for advanced detection and imaging applications. It is an essential tool for researchers and developers in the fields of bioconjugation, flow cytometry, and in vivo imaging, enabling high-sensitivity analysis across life sciences and diagnostics.

Application

• Antibody and Protein Labeling: Conjugation to monoclonal antibodies, proteins, and peptides for use in immunofluorescence, Western blotting, and ELISA.
• Nucleic Acid Probes: Labeling of oligonucleotides, DNA, and RNA for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Flow Cytometry & Cell Analysis: Preparation of fluorescent markers for cell surface receptor staining and intracellular target detection.
• In Vivo Imaging: Synthesis of near-infrared probes for deep-tissue imaging in preclinical animal studies due to minimal tissue autofluorescence.
• Microarray and Biosensor Development: Immobilization of biomolecules on solid surfaces for high-throughput screening and diagnostic devices.
• Drug Delivery & Targeting: Conjugation to nanoparticles, liposomes, or therapeutic agents for tracking biodistribution and pharmacokinetics.

Basic Information

Product Name	Cyanine5 NHS Ester
CAS No.	1032678-42-4
Molecular Formula	C37H42N3O5
Molecular Weight	608.75 g/mol
Synonyms	Cy5 NHS Ester; Sulfo-Cyanine5 NHS ester; Cy5 N-Hydroxysuccinimide ester; 1-(5-Carboxypentyl)-1'-ethyl-3,3,3',3'-tetramethylindocarbocyanine-5,5'-dicarboxylic acid bis(N-succinimidyl ester); Cy5® NHS ester; NHS-Cy5; Cyanine 5 carboxylic acid N-succinimidyl ester
EINECS	Contact for details

Quality Control

Our Cyanine5 NHS Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure consistent performance and batch-to-batch reproducibility. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark blue to purple solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Extinction Coefficient (ε)	≥ 250,000 cm⁻¹M⁻¹ at 646 nm
Solubility	Soluble in DMF, DMSO, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.