Description

3-Hydroxyacetaminophen-D3 is a stable isotope-labeled analog of a key metabolite of acetaminophen (paracetamol). This compound is of critical importance for use as an internal standard in quantitative bioanalytical methods, ensuring the highest accuracy and precision in mass spectrometry-based analyses. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, contract research organization (CRO), and clinical diagnostics sectors who require reliable quantification of acetaminophen and its metabolites in complex biological matrices.

Application

• Internal Standard for LC-MS/MS: Primary application as a deuterated internal standard for the accurate quantification of acetaminophen and 3-hydroxyacetaminophen in pharmacokinetic and toxicokinetic studies.
• Pharmaceutical R&D: Used in drug metabolism and pharmacokinetics (DMPK) research to study the metabolic pathways and clearance of acetaminophen.
• Clinical Research: Enables precise measurement of metabolite levels in plasma, serum, and urine for clinical trials investigating acetaminophen efficacy, safety, or overdose scenarios.
• Forensic Toxicology: Supports analytical methods for confirming and quantifying acetaminophen exposure in forensic casework.
• Biomarker Assay Development: Critical for developing and validating robust assays to monitor specific metabolites as potential biomarkers.
• Quality Control in Reference Material Production: Serves as a key component in the preparation and certification of certified reference materials (CRMs) for clinical chemistry.

Basic Information

Product Name	3-Hydroxyacetaminophen-D3
CAS No.	1020719-47-4
Molecular Formula	C8H6D3NO3
Molecular Weight	155.16 g/mol (for monoisotopic mass)
Synonyms	3-Hydroxyparacetamol-D3; N-(4-Hydroxyphenyl)-3-hydroxyacetamide-D3; 3'-Hydroxyacetaminophen-D3; Deuterated 3-Hydroxyacetaminophen; Acetaminophen Metabolite D3; [D3]-3-Hydroxyacetaminophen; 3-Hydroxy-4'-acetamidophenyl-D3; APAP Metabolite-d3
EINECS	Contact for details

Quality Control

Our 3-Hydroxyacetaminophen-D3 is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and mass spectrometry, to confirm identity, chemical purity, and isotopic enrichment. Certificates of Analysis (COA) detailing all relevant specifications are provided and can be tailored to meet specific project or regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label or COA. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Enrichment	≥98 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.