Description

Biotin-Peg4-Amide-C6-Azide is a specialized heterobifunctional crosslinker and bioconjugation reagent. This compound is engineered to facilitate the site-specific labeling and conjugation of biomolecules, combining the high-affinity binding of biotin with the bioorthogonal reactivity of an azide group via a flexible, hydrophilic PEG spacer. It is a critical tool for researchers and developers in the fields of proteomics, diagnostics, and targeted drug delivery who require precise and stable bioconjugates. The product is supplied under the CAS registry number 1006592-62-6.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of proteins, antibodies, and peptides for creating stable, functional conjugates.
• Diagnostic Assay Development: Used in the synthesis of probes and reagents for ELISA, lateral flow assays, and other immunoassay platforms.
• Chemical Biology & Proteomics: Enables pull-down assays, activity-based protein profiling (ABPP), and target identification via click chemistry with alkyne-bearing molecules.
• ADC (Antibody-Drug Conjugate) Research: Serves as a linker-payload component for constructing novel ADCs, leveraging the biotin-streptavidin interaction for purification or the azide for payload attachment.
• Cell Surface Engineering: Labeling of cell surface glycans or proteins for imaging, tracking, and cell sorting applications.
• Material Science & Nanotechnology: Functionalization of nanoparticles, surfaces, and hydrogels with bioactive ligands for biosensor and tissue engineering applications.
• Affinity Chromatography: Immobilization onto solid supports for the purification of streptavidin/avidin-tagged biomolecules.

Basic Information

Item	Detail
Product Name	Biotin-Peg4-Amide-C6-Azide
CAS No.	1006592-62-6
Molecular Formula	C31H55N7O9S
Molecular Weight	701.88 g/mol
Synonyms	Biotin-PEG4-C6-Azide; Biotin-PEG4-amide-C6-N3; 6-((6-(2-(2-(2-(2-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethoxy)ethoxy)ethoxy)ethoxy)ethyl)amino)-6-oxohexyl azide; Biotinamidohexanoic acid-PEG4-azide; N-(6-Azidohexyl)-23-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-6,9,12,15,18-pentaoxa-3-aza-23-oxotricosanamide; Biotin-PEG4-C6-N3; Azido-PEG4-Biotin
EINECS	Contact for details

Quality Control

Our Biotin-Peg4-Amide-C6-Azide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and MS (Mass Spectrometry) and NMR for structural confirmation, to ensure it meets the high standards required for research and development. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M+H]+
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.