Description

(3As,4S,6Ar)-Hexahydro-2-Oxo-n-(15-Oxo-3,6,9,12-Tetraoxa-16-Azanonadec-18-Yn-1-Yl)-1H-Thieno[3,4-D]Imidazole-4-Pentanamide is a sophisticated, high-purity synthetic intermediate featuring a biotin (vitamin B7) core conjugated with a polyethylene glycol (PEG) linker terminated with an alkyne functional group. This molecular architecture is engineered for precision bioconjugation, enabling the site-specific labeling and modification of biomolecules. It is primarily utilized by researchers and manufacturers in the pharmaceutical, diagnostic, and life science research sectors for developing targeted therapeutics, advanced imaging probes, and novel assay platforms.

Application

• ADC (Antibody-Drug Conjugate) Development: The alkyne handle allows for controlled, click chemistry-based conjugation of cytotoxic payloads to monoclonal antibodies.
• Diagnostic Probe Synthesis: Used to create biotinylated imaging agents (e.g., for MRI, fluorescence) with enhanced solubility and targeting via the PEG spacer.
• Affinity Chromatography Ligand: Serves as a key intermediate for synthesizing resins that purify streptavidin/avidin-binding proteins.
• Protein and Nucleic Acid Labeling: Facilitates the site-specific tagging of biomolecules for pull-down assays, western blotting, and cellular localization studies.
• Surface Functionalization: Employed to modify nanoparticles, biosensors, and microarray slides with biotin for subsequent capture and detection layers.
• Precision Medicine Research: Enables the development of targeted drug delivery systems and personalized diagnostic tools.

Basic Information

Product Name	(3As,4S,6Ar)-Hexahydro-2-Oxo-n-(15-Oxo-3,6,9,12-Tetraoxa-16-Azanonadec-18-Yn-1-Yl)-1H-Thieno[3,4-D]Imidazole-4-Pentanamide
CAS No.	1006592-45-5
Molecular Formula	C26H38N4O8S
Molecular Weight	566.67 g/mol
Synonyms	Biotin-PEG4-Alkyne; Biotin-PEG4-Propargyl; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(15-oxo-3,6,9,12-tetraoxa-16-azanonadec-18-yn-1-yl)pentanamide; Biotinamido-PEG4-Propargyl; Alkyne-PEG4-Biotin; Propargyl-PEG4-Biotin; N-(Biotinoyl)-N'-(propargyl-PEG4-amido)-1,4-diaminobutane (common naming); PEG4-Biotin Alkyne
EINECS	Contact for details

Quality Control

This high-value biochemical intermediate is produced under strict quality management systems. Each batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure structural integrity and purity. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity, identity, and impurity profiles. Our manufacturing aligns with cGMP principles where applicable, supporting its use in research and pre-clinical development phases.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.