Description

5-Bromo-3-Indoxyl-β-D-Glucuronic Acid Cyclohexylammonium Salt is a high-purity chromogenic substrate specifically designed for enzymatic detection and quantification. This compound is critical for research and diagnostic applications where precise and sensitive visualization of β-glucuronidase activity is required. It is primarily utilized by biotechnology firms, diagnostic kit manufacturers, and academic research institutions in life sciences.

Application

• Histochemistry & Cytochemistry: As a substrate for β-glucuronidase in tissue staining and cell-based assays, producing an insoluble blue indigo dye upon enzymatic cleavage.
• Microbiological Detection: For the identification and enumeration of coliform bacteria (e.g., E. coli) in water quality testing and food safety analysis, leveraging their specific β-glucuronidase activity.
• Molecular Biology Research: Used in reporter gene assays (e.g., GUS gene) to study gene expression and promoter activity in plant and microbial systems.
• Diagnostic Kit Manufacturing: A key component in the formulation of clinical and veterinary diagnostic kits for enzyme-linked detection.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential inhibitors or activators of β-glucuronidase.
• Biochemical Research: For the study of enzyme kinetics, inhibition, and the role of β-glucuronidase in various metabolic pathways.

Basic Information

Product Name	5-Bromo-3-Indoxyl-β-D-Glucuronic Acid Cyclohexylammonium Salt
CAS No.	199326-16-4
Molecular Formula	C20H27BrN2O8
Molecular Weight	503.34 g/mol
Synonyms	5-Bromo-3-indolyl-β-D-glucopyranosiduronic acid cyclohexylammonium salt; 5-Bromo-3-indoxyl-β-D-glucuronide cyclohexylammonium salt; X-GlucA, Cyclohexylammonium salt; 5-Br-3-Indoxyl-β-D-Glucuronic Acid, Cyclohexylamine Salt; 5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid cyclohexylammonium salt (common related compound); 5-Br-β-D-Glucuronide, Cyclohexylammonium salt; GUS Substrate, 5-Bromo derivative; β-Glucuronidase Substrate, 5-Bromo-3-indoxyl
EINECS	Contact for details

Quality Control

Our 5-Bromo-3-Indoxyl-β-D-Glucuronic Acid Cyclohexylammonium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccant use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines
Biological Activity	Suitable for enzyme assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.