Description

5-Bromo-6-Chloro-3-Indoxyl-α-D-Galactopyranoside is a specialized chromogenic substrate used for the enzymatic detection of α-galactosidase activity. This compound matters for its high sensitivity and specificity in biochemical assays, enabling precise research and diagnostic applications. It is primarily needed by biotechnology, pharmaceutical, and diagnostic kit manufacturing industries for applications in molecular biology, enzyme research, and microbiology.

Application

• Primary Substrate for α-Galactosidase Detection: Used in chromogenic assays to visualize and quantify α-galactosidase enzyme activity in research and diagnostic settings.
• Microbiology and Cell Biology: Employed in microbial identification and the study of gene expression (e.g., lacZ gene reporter systems) where α-galactosidase is used as a marker.
• Diagnostic Kit Manufacturing: A key component in the formulation of test kits for genetic disorders like Fabry disease, which involves α-galactosidase A deficiency.
• Food and Environmental Testing: Applied in assays to detect specific bacterial strains (e.g., E. coli) in food safety and water quality monitoring programs.
• Pharmaceutical Research: Utilized in high-throughput screening (HTS) to identify potential inhibitors or activators of the α-galactosidase enzyme for drug discovery.
• Histochemistry and IHC: Serves as a staining substrate in tissue sections for the localization of α-galactosidase activity.

Basic Information

Product Name	5-Bromo-6-Chloro-3-Indoxyl-α-D-Galactopyranoside
CAS No.	198402-60-7
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	5-Bromo-6-chloro-3-indolyl-α-D-galactopyranoside; X-α-Gal; 5-Bromo-6-chloro-1H-indol-3-yl α-D-galactopyranoside; BCIG; α-Galactosidase substrate; Chromogenic α-galactosidase substrate; 198402-60-7; 5-Br-6-Cl-3-Indoxyl-α-D-Galactoside
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indoxyl-α-D-Galactopyranoside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Heavy Metals	< 20 ppm
Residue on Ignition	< 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.