Description

Fluorescein Diphosphate Diammonium Salt is a high-purity, water-soluble enzymatic substrate used to generate the intensely fluorescent molecule fluorescein. This compound matters for its critical role as a sensitive and reliable probe in biochemical assays, particularly for detecting alkaline phosphatase activity. It is needed by researchers and manufacturers in the life sciences, diagnostic kit production, and pharmaceutical development for applications requiring precise enzymatic detection and quantification.

Application

• Alkaline Phosphatase (ALP) Assays: Primary substrate for fluorometric detection of ALP activity in ELISA, cell-based assays, and high-throughput screening.
• Diagnostic Reagent Manufacturing: Key component in the formulation of clinical diagnostic kits for measuring ALP levels, a biomarker for liver and bone disorders.
• Cell Biology & Histochemistry: Used as a non-radioactive, fluorescent probe for in situ localization of ALP activity in tissues and fixed cells.
• Biosensor Development: Integral to the design of optical biosensors and microarray platforms for environmental monitoring or pathogen detection.
• Pharmaceutical Research: Employed in drug discovery to screen for inhibitors or activators of alkaline phosphatase, a target for various therapeutic areas.
• Molecular Biology: Serves as a sensitive reporting system in nucleic acid hybridization assays when conjugated to oligonucleotide probes.

Basic Information

Product Name	Fluorescein Diphosphate Diammonium Salt
CAS No.	197777-66-5
Molecular Formula	C20H20N2O11P2 • xH2O
Molecular Weight	542.33 g/mol (Anhydrous)
Synonyms	Fluorescein Diphosphate, Diammonium Salt; FDP; Fluorescein Diphosphate, Ammonium Salt; 3',6'-Bis(dihydrogen phosphate)fluorescein diamide; ALP Substrate, Fluorogenic; FDDP; Fluorescein Bisphosphate Diammonium Salt; ELF 97 Substrate
EINECS	Contact for details

Quality Control

Our Fluorescein Diphosphate Diammonium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term storage under inert atmosphere, please contact us for specific recommendations.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Assay (Enzymatic)	≥ 90% (vs. standard)
Water Content (KF)	≤ 5.0%
Solubility (in Water)	Clear, yellow solution at 10 mg/mL
Fluorescence Background	Meets established limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.