Description

Wst-8 is a high-purity biochemical reagent widely used as a critical component in cell viability and proliferation assays. This compound, identified by CAS No. 193149-74-5, is essential for researchers and quality control laboratories requiring accurate and sensitive metabolic activity measurements. It is primarily utilized in the pharmaceutical, biotechnology, and academic research sectors for drug screening, cytotoxicity testing, and basic cell biology studies.

Application

• Cell Viability & Proliferation Assays: A key component in colorimetric assays (e.g., CCK-8 assays) to measure the number of viable cells in culture.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to evaluate the cytotoxic or proliferative effects of novel drug candidates.
• Cancer Research: Employed in studies to assess the sensitivity of cancer cell lines to chemotherapeutic agents and radiation therapy.
• Toxicology Testing: Applied in in vitro toxicology to determine the IC50 of environmental toxins or industrial chemicals on various cell types.
• Immunology Studies: Utilized to monitor lymphocyte proliferation and immune cell activation in response to stimuli.
• Biomaterial & Implant Testing: Helps evaluate the biocompatibility of new materials by assessing their impact on cell growth and metabolism.

Basic Information

Product Name	Wst-8
CAS No.	193149-74-5
Molecular Formula	C20H15N8NaO11S2
Molecular Weight	636.50 g/mol
Synonyms	WST-8; CCK-8 Reagent; 2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt; Water-Soluble Tetrazolium Salt 8; Cell Counting Kit-8 Dye; 5-Cyano-2,3-di-(4-nitrophenyl)-2H-tetrazolium hydroxide inner salt; Sodium 5-cyano-2,3-di(4-nitrophenyl)-2H-tetrazolium hydroxide inner salt
EINECS	Contact for details

Quality Control

Our Wst-8 is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, absorbance characteristics, and performance in standard cell-based assays. We ensure compliance with relevant quality benchmarks for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Absorbance (450 nm)	Characteristic
Solubility	Freely soluble in water
Biological Performance	Meets reference standard in cell assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.