Description

.Beta.-D-Glucofuranoside, 4-Nitrophenyl is a specialized synthetic glycoside derivative where a 4-nitrophenyl group is attached to the anomeric carbon of a D-glucofuranose ring. This compound is a critical chromogenic substrate used in enzymatic assays to detect and quantify the activity of β-glucosidase enzymes. It is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries who require precise and reliable tools for enzyme kinetics, inhibitor screening, and biochemical research.

Application

• Enzymatic Assay Substrate: Primary use as a chromogenic substrate for the spectrophotometric determination of β-glucosidase activity, where enzymatic hydrolysis releases yellow-colored 4-nitrophenol.
• Biochemical Research: Used in kinetic studies to measure enzyme activity, inhibition constants (Ki), and to characterize novel β-glucosidase enzymes.
• Diagnostic Reagent Manufacturing: Incorporated into reagent kits for clinical diagnostics to detect specific enzymatic deficiencies or for microbial identification.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify potential β-glucosidase inhibitors for drug discovery programs.
• Quality Control (QC) Testing: Used in QC labs to standardize and validate the performance of β-glucosidase enzymes used in various industrial processes.
• Plant & Microbial Biochemistry: Applied in research to study cellulose degradation pathways in fungi and bacteria, as β-glucosidase is a key cellulase enzyme.

Basic Information

Product Name	.Beta.-D-Glucofuranoside, 4-Nitrophenyl
CAS No.	192717-86-5
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	4-Nitrophenyl β-D-Glucofuranoside; p-Nitrophenyl β-D-Glucofuranoside; PNPGf; β-D-Glucofuranosyl 4-nitrobenzoate; 4-Nitrophenyl-β-D-glucofuranoside; NPG; 1-O-(4-Nitrophenyl)-β-D-glucofuranose
EINECS	Contact for details

Quality Control

Our .Beta.-D-Glucofuranoside, 4-Nitrophenyl is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry guidelines to support your compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is kept tightly sealed in a desiccated environment to maintain stability and prevent decomposition.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.