Description

Pyr-His-Pro-Amc CAS NO 190836-86-3 is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of specific protease activity. It is primarily utilized in pharmaceutical R&D, biochemical research, and diagnostic kit development for studying enzyme kinetics and inhibitor screening.

Application

• Protease Activity Assays: Fluorogenic substrate for the quantitative determination of specific protease enzyme activity in research and diagnostic applications.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) campaigns to identify and characterize novel protease inhibitors as potential drug candidates.
• Biochemical Pathway Analysis: A tool for studying the role and regulation of specific proteases in complex biological processes and disease mechanisms.
• Diagnostic Kit Manufacturing: A key component in the formulation of clinical diagnostic tests that measure protease levels as biomarkers.
• Enzyme Kinetics Studies: Enables precise measurement of kinetic parameters (Km, Vmax) for target proteases due to its fluorogenic properties.
• Quality Control in Biologics: Used to monitor and control protease activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	Pyr-His-Pro-Amc
CAS No.	190836-86-3
Molecular Formula	C25H30N8O5
Molecular Weight	522.56 g/mol
Synonyms	Pyroglutamyl-Histidyl-Prolyl-7-Amino-4-Methylcoumarin; Pyr-His-Pro-AMC; pGlu-His-Pro-AMC; Substrate for TRH-Degrading Ectoenzyme; Fluorogenic TRH Substrate; EC 3.4.19.6 Substrate; Pyroglutamyl-Histidyl-Proline-AMC; L-Pyroglutamyl-L-histidyl-L-prolyl-7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Every batch of Pyr-His-Pro-Amc is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and performance consistency. Our quality commitment includes comprehensive analysis via HPLC, MS, and NMR. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.