Description

1H-Thieno[3,4-D]Imidazole-4-Pentanoic Acid, Hexahydro-2-Oxo-, 2,5-Dioxo-3-Sulfo-1-Pyrrolidinyl Ester, (3As,4S,6Ar)- CAS NO 190598-55-1 is a high-purity, specialized heterocyclic compound featuring a reactive N-hydroxysuccinimide (NHS) ester group. This molecular structure makes it a critical intermediate for bioconjugation and crosslinking applications, enabling the formation of stable amide bonds with primary amines under mild conditions. It is primarily sought after by pharmaceutical researchers, biotech companies, and diagnostic reagent manufacturers for developing advanced therapeutic agents, antibody-drug conjugates (ADCs), and sensitive assay systems.

Application

• Bioconjugation Reagent: A key building block for covalently linking biomolecules (e.g., proteins, peptides, antibodies) to drugs, fluorophores, or solid supports.
• Antibody-Drug Conjugate (ADC) Development: Used as a linker-payload intermediate to create targeted cancer therapies.
• Diagnostic Assay Manufacturing: Employed in the synthesis of enzyme conjugates and labeled antigens/antibodies for ELISA, lateral flow tests, and other immunoassays.
• Polymer Functionalization: For modifying surfaces of nanoparticles, hydrogels, or chromatography media with active ester groups for further coupling.
• Peptide Synthesis & Modification: Used in solid-phase peptide synthesis (SPPS) or for site-specific modification of synthetic peptides.
• Proteomics Research: As a tool for chemical proteomics, including activity-based protein profiling (ABPP) and cross-linking mass spectrometry studies.
• Material Science: Surface engineering of biomaterials to enhance biocompatibility or introduce specific functional groups.

Basic Information

Product Name	1H-Thieno[3,4-D]Imidazole-4-Pentanoic Acid, Hexahydro-2-Oxo-, 2,5-Dioxo-3-Sulfo-1-Pyrrolidinyl Ester, (3As,4S,6Ar)-
CAS No.	190598-55-1
Molecular Formula	C15H19N3O8S2
Molecular Weight	433.45 g/mol
Synonyms	N-Sulfosuccinimidyl Biotinylate; Biotin-XX, SSE; Sulfo-NHS-Biotin-XX; Biotin-XX sulfosuccinimidyl ester; 6-((6-((2-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)ethyl)amino)-6-oxohexanoyl)amino)hexanoic acid, 2,5-dioxopyrrolidin-1-yl ester, 3-sulfo; Biotinamidohexanoic acid sulfosuccinimidyl ester; Biotin-LC-LC-NHS; EZ-Link Sulfo-NHS-Biotin-XX
EINECS	Contact for details

Quality Control

Our 1H-Thieno[3,4-D]Imidazole-4-Pentanoic Acid, Hexahydro-2-Oxo-, 2,5-Dioxo-3-Sulfo-1-Pyrrolidinyl Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional activity testing. Certificates of Analysis (COA) detailing all specifications and test results are provided to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, desiccate under an inert atmosphere. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO, DMF
Functional Activity	Conforms (by amine coupling assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.