Description

Mca-Asp-Glu-Val-Asp-Ala-Pro-Lys(Dnp)-Oh is a specialized fluorogenic peptide substrate designed for the sensitive detection and study of caspase activity, particularly caspase-3 and related enzymes. This compound is critical for researchers and developers in the life sciences and pharmaceutical industries who require precise tools for apoptosis research and drug discovery. Its high specificity and sensitivity make it an essential reagent for biochemical assays, high-throughput screening, and the development of diagnostic kits.

Application

• Caspase-3 Activity Assays: Primary substrate for fluorometric and spectrophotometric determination of caspase-3 enzyme activity in cell lysates and purified systems.
• Apoptosis Research: Used to study programmed cell death pathways in cancer research, neurobiology, and immunology.
• High-Throughput Screening (HTS): Enables the screening of compound libraries for potential modulators (inhibitors or activators) of caspase activity in drug discovery.
• Diagnostic Kit Development: Serves as a key component in the development of commercial assay kits for research and clinical diagnostics.
• Biochemical Characterization: Employed in enzyme kinetics studies to determine Km, Vmax, and inhibition constants (Ki) for caspase enzymes.
• Quality Control in Biopharma: Used in the QC testing of biopharmaceutical products where apoptosis is a critical quality attribute.

Basic Information

Product Name	Mca-Asp-Glu-Val-Asp-Ala-Pro-Lys(Dnp)-Oh
CAS No.	189696-20-6
Molecular Formula	C52H70N12O22
Molecular Weight	1231.18 g/mol
Synonyms	Ac-DEVD-AFC; Caspase-3 Substrate I (Ac-DEVD-AFC); Ac-Asp-Glu-Val-Asp-7-amino-4-trifluoromethylcoumarin; Ac-DEVD-AFC Caspase Substrate; Fluorogenic Caspase-3 Substrate; MCA-DEVDAPK(DNP)-OH; (7-Methoxycoumarin-4-yl)acetyl-Asp-Glu-Val-Asp-Ala-Pro-Lys(2,4-dinitrophenyl)-OH; Caspase 3 Fluorogenic Substrate
EINECS	Contact for details

Quality Control

Our Mca-Asp-Glu-Val-Asp-Ala-Pro-Lys(Dnp)-Oh is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and mass spectrometry for identity confirmation. Certificates of Analysis (COA) detailing purity, assay, and related substance profiles are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, keep desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 98% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.