Description

2-Chloro-4-Nitrophenyl-B-D-Cellotetraoside is a specialized chromogenic substrate designed for the sensitive and specific detection of cellulase enzyme activity. This compound matters as a critical tool for researchers and quality control labs requiring precise quantification of cellulolytic activity in complex samples. It is primarily needed by the biotechnology, biofuel research, and industrial enzyme production sectors for applications in enzymatic assay development and biochemical research.

Application

• Cellulase Enzyme Assays: Acts as a specific chromogenic substrate for the colorimetric determination of endo-1,4-β-glucanase (cellulase) activity.
• Biomass Conversion Research: Used in studying and optimizing enzymatic hydrolysis of cellulose for biofuel (e.g., ethanol) production.
• Enzyme Kinetics Studies: Enables the measurement of reaction rates and inhibition studies for cellulase enzymes.
• Quality Control in Enzyme Production: Employed to standardize and batch-test cellulase preparations from microbial or fungal sources.
• Diagnostic Reagent Manufacturing: Serves as a key component in diagnostic kits for detecting specific cellulolytic microorganisms.
• Agricultural Research: Applied in studies of plant-pathogen interactions where cellulase is a virulence factor.
• Textile and Pulp & Paper Industries: Used in R&D for evaluating the effectiveness of enzymatic treatments and bio-polishing processes.

Basic Information

Product Name	2-Chloro-4-Nitrophenyl-B-D-Cellotetraoside
CAS No.	189094-93-7
Molecular Formula	C30H41ClN2O26
Molecular Weight	833.09 g/mol
Synonyms	4-Nitrophenyl 2-chloro-4-nitrophenyl β-D-cellotetraoside; 2-Chloro-4-nitrophenyl β-cellotetraoside; CNP-Cellotetraoside; β-4-Nitrophenyl 2-chloro-4-nitrophenyl cellotetraoside; 2-Chloro-4-nitrophenyl-β-D-cellotetraose; Chromogenic cellulase substrate; Cellulase substrate CNP-C4; p-Nitrophenyl 2-chloro-4-nitrophenyl β-D-cellotetraoside
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for biochemical reagents. Each batch is characterized using advanced analytical techniques to guarantee identity, purity, and performance in enzymatic assays. Certificates of Analysis (COA) detailing purity, assay performance, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term stability, storage at -20°C is recommended. Allow the product to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Assay (Enzymatic Activity)	Conforms to performance standard
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.