Description

Bcip Dipotassium Salt is a high-purity biochemical reagent widely used as a chromogenic substrate in enzymatic assays. This compound is critical for research and diagnostic applications requiring precise colorimetric detection of alkaline phosphatase activity. It is an essential tool for scientists and quality control professionals in the pharmaceutical, biotechnology, and life science research sectors.

Application

• Enzymatic Assay Substrate: Primary use as a chromogenic substrate for detecting alkaline phosphatase (ALP) activity in ELISA, Western blotting, and other immunoassays.
• Molecular Biology Research: Used in nucleic acid hybridization techniques, including Southern and Northern blotting, for non-radioactive detection.
• Diagnostic Kit Manufacturing: A key raw material in the production of clinical diagnostic kits for various diseases and conditions.
• Cell & Tissue Staining: Employed in histochemistry and cytochemistry for visualizing enzyme activity in biological samples.
• Pharmaceutical QC Testing: Utilized in quality control laboratories for testing and validation within biopharmaceutical production processes.
• Food Safety & Environmental Testing: Applied in assays designed to detect bacterial contamination or specific enzymatic markers.

Basic Information

Product Name	Bcip Dipotassium Salt
CAS No.	185335-30-2
Molecular Formula	C14H15BrClN5O4K2
Molecular Weight	519.38 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt dipotassium; BCIP dipotassium salt; 5-Bromo-4-chloro-3-indolyl phosphate, dipotassium salt; X-Phosphate dipotassium salt; BCIP, K2 salt; Alkaline Phosphatase Substrate, BCIP; 5-Bromo-4-chloro-3-indolyl phosphate potassium salt
EINECS	Contact for details

Quality Control

Our Bcip Dipotassium Salt CAS NO 185335-30-2 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, identity confirmation by IR and NMR spectroscopy, and stringent controls for moisture and heavy metal content. Certificates of Analysis (COA) with full traceability are provided to ensure compliance with research and diagnostic-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term stability, store desiccated under inert atmosphere. Keep container tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Heavy Metals (as Pb)	≤ 20 ppm
Solubility (Test)	Clear, colorless solution in water
Functional Test (Enzymatic)	Pass

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.