Description

n-(13-Amino-4,7,10-Trioxatridecanyl)-D-Biotinamide is a specialized biotin derivative featuring a hydrophilic polyether spacer arm terminated with a primary amine group. This molecular design is critical for creating stable conjugates, enabling the biotin-avidin/streptavidin interaction to be leveraged in advanced diagnostic and research applications. It is an essential intermediate for biochemists and pharmaceutical researchers developing sensitive assay systems, targeted drug delivery platforms, and novel biomaterials.

Application

• Bioconjugation & Labeling Reagent: Primary amine group facilitates covalent attachment to carboxylates, carbonyls, or activated surfaces for introducing biotin tags onto proteins, antibodies, oligonucleotides, and nanoparticles.
• Diagnostic Assay Development: Used in the synthesis of reagents for ELISA, lateral flow assays, and other immunoassays that utilize the high-affinity streptavidin-biotin system for signal amplification.
• Affinity Purification Systems: Serves as a key component in creating ligands for immobilization on solid supports (e.g., agarose beads) to purify streptavidin/avidin-tagged biomolecules.
• Drug Delivery & Targeting: Enables the biotinylation of therapeutic agents or carriers for targeted delivery strategies exploiting overexpressed receptors or pre-targeting approaches.
• Surface Functionalization: The amine-terminated linker allows for the modification of biosensor chips, microarray slides, and other substrates to create bioactive surfaces.
• Molecular Probes & Imaging Agents: Intermediate in the synthesis of biotinylated fluorescent dyes, MRI contrast agents, or radioligands for in vitro and in vivo imaging.

Basic Information

Product Name	n-(13-Amino-4,7,10-Trioxatridecanyl)-D-Biotinamide
CAS No.	183896-00-6
Molecular Formula	C22H42N4O6S
Molecular Weight	490.66 g/mol
Synonyms	Biotin-PEG3-amine; Biotin-PEG3-NH2; D-Biotin-PEG3-Amine; Biotinamido-PEG3-amine; (+)-Biotin-3,6,9-trioxaundecanediamine; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-[2-[2-(2-aminoethoxy)ethoxy]ethyl]pentanamide; Biotinamido-PEG3-amine; Biotin-PEG3-NH2 TFA salt (common salt form)
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research and diagnostic use. Each batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and correct molecular structure. Certificates of Analysis (COA) detailing all relevant specifications are available upon request. We adhere to a quality management system to ensure batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This compound is hygroscopic (moisture-sensitive); containers should be sealed under inert atmosphere or with desiccant after opening to prevent degradation. For long-term storage (over 12 months), consider storing at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.