Description

Sulfosuccinimidyl n-[N'-(D-Biotinyl)-6-Aminohexanoyl]-6'-Aminohexanoate is a heterobifunctional crosslinking reagent designed for the bioconjugation of biomolecules. This compound is critically important for creating stable, site-specific linkages between proteins, peptides, and other amine-containing molecules and biotin tags, enabling downstream purification or detection via streptavidin. It is an essential tool for researchers and developers in biopharmaceutical R&D, diagnostic assay development, and proteomics, where precise and reliable labeling is paramount.

Application

• Bioconjugation and Protein Labeling: Primary reagent for covalently attaching biotin to antibodies, enzymes, and other proteins for use in ELISA, Western blotting, and flow cytometry.
• Diagnostic Kit Manufacturing: Used in the development and production of immunoassays and biosensors that require a stable biotin-streptavidin detection system.
• Affinity Chromatography Ligand Preparation: Immobilization of biotinylated ligands onto streptavidin or avidin resins for the purification of target molecules.
• Cell Surface Engineering: Labeling of cell surface proteins or receptors with biotin for tracking, isolation, or functional studies.
• Proteomics and Biomarker Discovery: Facilitating pull-down assays and mass spectrometry workflows by biotinylating bait proteins or peptides.
• Drug Delivery System Development: Conjugation of targeting ligands (e.g., antibodies) to drug carriers or nanoparticles via a biotin bridge.

Basic Information

Product Name	Sulfosuccinimidyl n-[N'-(D-Biotinyl)-6-Aminohexanoyl]-6'-Aminohexanoate
CAS No.	180028-78-8
Molecular Formula	C30H48N6O10S
Molecular Weight	684.80 g/mol
Synonyms	Sulfo-NHS-LC-LC-Biotin; Biotin-(AC5)2-Sulfo-OSu; N-ε-Biotinyl-N-α-(3-sulfo-N-succinimidyloxycarbonylpropyl)-L-lysyl-L-lysine; Biotin-LC-LC-Sulfo-OSu; Biotin-XX, sulfosuccinimidyl ester; Biotin (Long Chain) Sulfosuccinimidyl Ester; EZ-Link Sulfo-NHS-LC-LC-Biotin; 3-({6-[(6-{5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)hexanoyl]amino}hexanoyl)-N-oxysulfosuccinimide sodium salt
EINECS	Contact for details

Quality Control

Our Sulfosuccinimidyl n-[N'-(D-Biotinyl)-6-Aminohexanoyl]-6'-Aminohexanoate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed specifications are provided for every lot to guarantee traceability and performance consistency.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO, DMF
Functional Activity	Conforms (Biotin incorporation assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.