Description

2-Benzothiazolyl-3-(4-Carboxy-2-Methoxyphenyl)-5-(4-(2-Sulfoethylcarbamoyl)Phenyl)-2H-Tetrazolium is a specialized tetrazolium salt derivative with a complex, functionalized structure designed for high-performance biochemical assays. This compound matters for its critical role as a sensitive and stable chromogenic substrate in enzymatic detection systems, particularly for dehydrogenase and reductase activity. It is primarily needed by manufacturers of diagnostic kits, research institutions, and pharmaceutical companies engaged in cell viability testing, cytotoxicity screening, and metabolic activity studies. Its unique structure provides enhanced water solubility and signal generation compared to simpler tetrazolium salts.

Application

• Cell Viability & Proliferation Assays (e.g., MTT alternative): Used as a key substrate in colorimetric assays to measure mitochondrial activity in live cells.
• Diagnostic Reagent Manufacturing: Incorporated into clinical diagnostic kits for the detection of specific enzymes or biomarkers.
• Cytotoxicity & Drug Screening: Employed in high-throughput screening (HTS) platforms in pharmaceutical R&D to evaluate the effects of compounds on cell health.
• Biochemical Research: Serves as an electron acceptor in dehydrogenase-coupled reaction systems for studying metabolic pathways.
• Microbiology & Cell Biology: Applied in assays to monitor bacterial growth or fungal contamination.
• Histochemistry & Staining: Potential use in tissue staining protocols to localize enzymatic activity.

Basic Information

Product Name	2-Benzothiazolyl-3-(4-Carboxy-2-Methoxyphenyl)-5-(4-(2-Sulfoethylcarbamoyl)Phenyl)-2H-Tetrazolium
CAS No.	178925-54-7
Molecular Formula	C32H25N7O8S2
Molecular Weight	699.71 g/mol
Synonyms	WST-5; 2-(4-Carboxy-2-methoxyphenyl)-3-(4-(2-sulfoethylcarbamoyl)phenyl)-5-(2-benzothiazolyl)-2H-tetrazolium; Tetrazolium Salt WST-5; 2H-Tetrazolium, 2-(2-benzothiazolyl)-3-[4-(carboxy)-2-methoxyphenyl]-5-[4-[(2-sulfoethyl)carbamoyl]phenyl]-; Water-soluble tetrazolium salt 5; Benzothiazolyl tetrazolium carboxyl sulfophenyl derivative
EINECS	Contact for details

Quality Control

Our 2-Benzothiazolyl-3-(4-Carboxy-2-Methoxyphenyl)-5-(4-(2-Sulfoethylcarbamoyl)Phenyl)-2H-Tetrazolium is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectrophotometry for absorbance characteristics, and identity confirmation via IR and NMR, to ensure it meets the stringent requirements for diagnostic and research applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent absorption of moisture, which can affect performance and stability. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Absorbance (λ max)	Conforms to reference
Solubility (in Water)	Clear, yellow solution at 10 mg/mL
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.