Description

5-Bromo-4-Chloro-3-Indolyl β-D-Cellobioside is a chromogenic substrate specifically designed for the detection of cellulase and β-glucosidase enzyme activity. This compound is critical for research and industrial applications requiring precise enzymatic analysis and quantification. It is primarily used by biotechnologists, enzymologists, and quality control laboratories in the biofuel, pulp & paper, textile, and food processing industries.

Application

• Enzyme Activity Assays: As a specific chromogenic substrate for the detection and quantification of cellulase and β-glucosidase activity in research and diagnostic kits.
• Biomass Conversion Research: Used in high-throughput screening to identify and characterize novel cellulolytic enzymes for biofuel production from lignocellulosic materials.
• Microbiology & Diagnostics: Employed in microbial identification media to detect cellulase-producing microorganisms, such as certain fungi and bacteria.
• Industrial Process Monitoring: Applied in quality control within industries like textiles, detergents, and animal feed to monitor the activity of commercial enzyme preparations.
• Plant & Agricultural Research: Utilized to study plant cell wall degradation and the role of cellulases in plant-pathogen interactions.

Basic Information

Item	Details
Product Name	5-Bromo-4-Chloro-3-Indolyl β-D-Cellobioside
CAS No.	177966-52-8
Molecular Formula	C20H25BrClNO11
Molecular Weight	562.77 g/mol
Synonyms	X-Cellobioside; 5-Bromo-4-chloro-3-indolyl β-D-cellobioside; BCIP-Cellobioside; Indoxyl-β-D-cellobioside, 5-Bromo-4-chloro-; Chromogenic Cellobioside Substrate; 3-Indolyl β-D-cellobioside, 5-bromo-4-chloro-; BCI-Cellobioside; 177966-52-8
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl β-D-Cellobioside is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency suitable for sensitive enzymatic assays. Each lot undergoes rigorous analytical testing, including HPLC for purity and spectroscopic identification. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.