Description

5-Cyano-2,3-Bis(4-Nitrophenyl)-2H-Tetrazolium Chloride is a specialized tetrazolium salt derivative, primarily valued for its role as a critical reagent in biochemical assays. Its core value proposition lies in its ability to serve as a sensitive and reliable electron acceptor in the detection of cellular metabolic activity, particularly in cell viability and cytotoxicity testing. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, where precise measurement of dehydrogenase activity is required.

Application

• Cell Viability and Proliferation Assays: Used as a key substrate in colorimetric assays like MTT and related tests to measure mitochondrial activity in live cells.
• Cytotoxicity Screening: Employed in high-throughput screening platforms for evaluating the toxic effects of drug candidates, chemicals, or nanomaterials on cultured cells.
• Microbiology Research: Applied in assays to study microbial dehydrogenase activity and metabolic function in bacteria and yeast.
• Biochemical Research: Serves as an electron acceptor in enzymatic assays for various oxidoreductases, facilitating spectrophotometric analysis.
• Pharmaceutical Quality Control: Utilized in in-process testing and stability studies to assess the biological activity and potential cytotoxicity of pharmaceutical formulations.
• Diagnostic Reagent Formulation: A component in the development of diagnostic kits for clinical and research applications requiring metabolic endpoint detection.

Basic Information

Product Name	5-Cyano-2,3-Bis(4-Nitrophenyl)-2H-Tetrazolium Chloride
CAS No.	176255-37-1
Molecular Formula	C20H12ClN7O4
Molecular Weight	449.81 g/mol
Synonyms	5-Cyano-2,3-di(4-nitrophenyl)-2H-tetrazolium chloride; 2H-Tetrazolium, 5-cyano-2,3-bis(4-nitrophenyl)-, chloride; Tetrazolium Chloride Derivative; Nitro Tetrazolium Blue Derivative; CNTZ; 5-CN-2,3-DNTT; 2-(4-Nitrophenyl)-3-(4-nitrophenyl)-5-cyanotetrazolium chloride
EINECS	Contact for details

Quality Control

Our 5-Cyano-2,3-Bis(4-Nitrophenyl)-2H-Tetrazolium Chloride is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to prevent degradation. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals (as Pb)	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.