Description

2-[N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Amino]-2-Deoxy-D-Glucose is a fluorescently labeled glucose derivative, specifically a 2-deoxyglucose analog conjugated with the NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl) fluorophore. This compound is a critical tool for real-time visualization and quantification of glucose uptake and metabolism in living cells and tissues. It is primarily utilized by researchers and scientists in the fields of cell biology, oncology, and metabolic disease research to study cellular energetics, tumor metabolism, and drug effects on glucose transport mechanisms.

Application

• Cellular Glucose Uptake Assays: Direct visualization and quantification of glucose transport activity in live cells using fluorescence microscopy or flow cytometry.
• Cancer Metabolism Research: Investigating the Warburg effect and altered metabolic pathways in tumor cells for drug discovery and diagnostic development.
• Neuroscience Studies: Mapping neuronal activity and brain metabolism based on regional glucose utilization.
• Metabolic Disorder Modeling: Studying insulin resistance, diabetes, and other diseases affecting glucose homeostasis in cell-based models.
• High-Content Screening (HCS): Serving as a key reagent in automated screening platforms to identify compounds that modulate glucose metabolism.
• Microbial Metabolism: Probing sugar transport and utilization in bacteria and yeast for microbiological and biotechnological applications.

Basic Information

Product Name	2-[N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Amino]-2-Deoxy-D-Glucose
CAS No.	174844-42-9
Molecular Formula	C₁₄H₁₆N₄O₈
Molecular Weight	368.30 g/mol
Synonyms	2-NBDG; 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose; NBD 2-Deoxy-D-glucose; 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose; 2-Deoxy-2-[(7-nitro-4-benzofurazanyl)amino]-D-glucose; Fluorescent D-glucose analog; NBDG; Glucose Uptake Probe
EINECS	Contact for details

Quality Control

Our 2-NBDG is produced under strict quality management protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity verification and spectroscopic methods for identity confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, fluorescence properties, and absence of critical impurities. We support research compliance and data integrity with full traceability.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. The product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Fluorescence Properties	Ex/Em ~465/540 nm (in aqueous buffer)
Biological Activity	Competitively inhibits 2-Deoxy-D-glucose uptake

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.