Description

Cyclophosphamide-D4 CAS NO 173547-45-0 is a deuterated analog of the widely used alkylating agent cyclophosphamide, where four hydrogen atoms are replaced with deuterium. This stable isotope-labeled compound is essential for precise pharmacokinetic and metabolic studies, serving as a critical internal standard in quantitative bioanalytical methods. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in drug metabolism and disposition (DMPK) research, clinical trial analysis, and forensic toxicology.

Application

• Internal Standard for LC-MS/MS: Used as a quantitative reference standard in liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays for cyclophosphamide and its metabolites in biological matrices.
• Drug Metabolism Studies (DMPK): Facilitates accurate tracking and quantification of parent drug and metabolite levels in in vitro and in vivo studies.
• Clinical Pharmacology & Therapeutic Drug Monitoring (TDM): Enables precise measurement of cyclophosphamide concentrations in patient plasma or serum to optimize dosing regimens.
• Bioanalytical Method Development & Validation: Critical for developing robust, sensitive, and specific analytical methods compliant with FDA, EMA, and ICH guidelines.
• Forensic & Toxicological Analysis: Used in forensic laboratories for the definitive identification and quantification of cyclophosphamide in complex samples.
• Stable Isotope Dilution Assays: Employed in high-precision assays where deuterium labeling minimizes matrix effects and improves analytical accuracy.

Basic Information

Item	Detail
Product Name	Cyclophosphamide-D4
CAS No.	173547-45-0
Molecular Formula	C7H11D4Cl2N2O2P
Molecular Weight	265.12 g/mol (approx.)
Synonyms	Cyclophosphamide-d4; 2-[Bis(2-chloroethyl)amino]tetrahydro-2H-1,3,2-oxazaphosphorine 2-oxide-d4; N,N-Bis(2-chloroethyl)-N',O-propylenephosphoric acid ester diamide-d4; Deuterated Cyclophosphamide; Cyclophosphamide (D4); CP-D4; Endoxan-d4; Cytoxan-d4
EINECS	Contact for details

Quality Control

Our Cyclophosphamide-D4 is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and MS, to confirm identity, chemical purity, and deuterium enrichment. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Purity (D-enrichment)	≥98 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.