Description

5-Bromo-4-Chloro-3-Indoxyl-α-L-Fucopyranoside is a specialized chromogenic substrate used for the specific detection of α-L-fucosidase enzyme activity. This compound is critical for research and diagnostic applications where precise enzymatic detection is required. It is primarily utilized by biochemists, diagnostic kit manufacturers, and life science researchers working in enzymology, histochemistry, and microbiology.

Application

• Enzymatic Substrate for α-L-Fucosidase: Used as a key reagent in biochemical assays to detect and quantify α-L-fucosidase activity, an enzyme relevant in various metabolic studies.
• Diagnostic Kit Manufacturing: Incorporated into clinical diagnostic kits for the detection of specific enzyme deficiencies or bacterial identification.
• Microbiological Research: Employed in microbial culture media for the differentiation and identification of bacterial species based on their enzymatic profiles.
• Histochemistry and Cell Biology: Acts as a chromogenic agent in staining procedures to localize α-L-fucosidase activity in tissue sections and cell cultures.
• Biochemical Research Reagent: Serves as a high-purity substrate in academic and industrial research focused on glycosidase enzymes and carbohydrate metabolism.
• Quality Control in Pharmaceutical Development: Used in the development and validation of assays for biopharmaceutical products where specific glycosidase activities must be monitored.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indoxyl-α-L-Fucopyranoside
CAS No.	171869-92-4
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl-α-L-fucopyranoside; X-α-L-Fuc; BCIP-α-L-Fuc; 5-Br-4-Cl-3-indoxyl-α-L-fucoside; α-L-Fucosidase substrate; Chromogenic α-L-fucopyranoside; Indoxyl-α-L-fucopyranoside derivative; 171869-92-4
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indoxyl-α-L-Fucopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and spectroscopic identification. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.