Description

Tuberous Sclerosis 2 Protein CAS NO 169027-60-5 is a highly purified recombinant protein, also known as Tuberin, which is a critical component of the TSC1-TSC2 protein complex. This protein is essential for research into the molecular mechanisms of tuberous sclerosis complex (TSC), a genetic disorder, and its associated signaling pathways, particularly the mTOR pathway. It is primarily utilized by researchers and developers in academic institutions, pharmaceutical R&D, and biotechnology companies focused on oncology, neurology, and rare disease therapeutics.

Application

• Biomedical Research: Used as a key reagent in fundamental research to study the function and regulation of the TSC1/TSC2 complex and its role in cell growth, proliferation, and tumor suppression.
• Drug Discovery & Development: Serves as a target protein for screening and validating potential therapeutic compounds aimed at modulating the mTOR pathway for cancer and TSC treatment.
• Diagnostic Assay Development: Employed in the development of immunoassays (ELISA, Western Blot) for the detection and quantification of Tuberin or related biomarkers.
• Enzymatic Activity Studies: Utilized in in vitro studies to investigate the GTPase-activating protein (GAP) activity of Tuberin towards Rheb (Ras homolog enriched in brain).
• Protein-Protein Interaction Analysis: Acts as a bait or prey protein in pull-down assays, co-immunoprecipitation, or surface plasmon resonance (SPR) to map interaction networks.
• Antigen for Antibody Production: Provides a high-purity immunogen for generating specific polyclonal or monoclonal antibodies against Tuberous Sclerosis 2 Protein.
• Cell Signaling Pathway Analysis: A critical tool for elucidating the PI3K/Akt/mTOR signaling cascade and its dysregulation in various diseases.
• Quality Control Reference Standard: Used as a calibrated standard in QC laboratories to ensure the consistency and accuracy of diagnostic kits or other research reagents.

Basic Information

Item	Details
Product Name	Tuberous Sclerosis 2 Protein
CAS No.	169027-60-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Tuberin; TSC2 Protein; Tuberous Sclerosis Complex Protein 2; Tuberous Sclerosis 2 Tumor Suppressor; LAM Protein; PPP1R160; TSC2; Recombinant Human Tuberin
EINECS	Contact for details

Quality Control

Our Tuberous Sclerosis 2 Protein is produced under stringent conditions to ensure the highest levels of purity and biological activity. Each batch undergoes rigorous quality testing, including SDS-PAGE, Western Blot analysis, and functional activity assays, to guarantee consistency and performance for critical research applications. Certificates of Analysis (COA) detailing purity, concentration, and specific activity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (SDS-PAGE)	Single band at expected molecular weight
Identification (Western Blot)	Positive reactivity with specific anti-TSC2 antibody
Purity (HPLC)	≥ 95%
Protein Content	Determined by UV absorbance or colorimetric assay
Biological Activity	Confirmed by functional assay (e.g., GAP activity towards Rheb)
Endotoxin Level (LAL)	< 1.0 EU/μg
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.