Description

Thioglo(Tm)-5* CAS NO 168639-87-0 is a high-purity, specialized chemical reagent designed for sensitive analytical and synthetic applications. Its primary value lies in its role as a selective fluorogenic probe for the detection and quantification of thiols, offering high sensitivity and specificity. This product is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic kit manufacturing sectors who require reliable tools for biochemical analysis and assay development.

Application

• Fluorogenic labeling agent for the detection of low-molecular-weight thiols (e.g., glutathione, cysteine) in biological samples.
• Key component in the development of high-throughput screening (HTS) assays for enzyme activity related to thiol metabolism.
• Used in research for studying oxidative stress and redox status in cell cultures and tissue samples.
• Critical reagent in the formulation of diagnostic kits for clinical analysis of thiol levels.
• Tool for probing protein thiol groups in structural biology and proteomics research.
• Quality control testing in pharmaceutical manufacturing for thiol-containing drug substances.

Basic Information

Product Name	Thioglo(Tm)-5* CAS NO 168639-87-0
CAS No.	168639-87-0
Molecular Formula	C23H21N3O6S2
Molecular Weight	499.56 g/mol
Synonyms	Thioglo-5; 5-Maleimidylfluorescein; 5-MF; MF; 5-((2-(and-3)-S-(acetylmercapto)succinoyl)amino)fluorescein; Fluorescein-5-maleimide; N-(Fluorescein-5-yl)maleimide; Thiol detection reagent 5
EINECS	Contact for details

Quality Control

Every batch of Thioglo(Tm)-5* is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure high purity and consistent performance. Our quality assurance protocol includes identity confirmation, purity assay, and verification of fluorogenic activity. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with each shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Fluorescence Properties	Ex/Em ~490/515 nm (upon thiol reaction)
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.