Description

Sodium 4-[3-(4-Iodophenyl)-2-(2,4-Dinitrophenyl)-2H-5-Tetrazolio]-1,3-Benzene Disulfonate is a specialized tetrazolium salt derivative, primarily valued for its role as a vital chromogenic reagent in biochemical and analytical applications. Its core value proposition lies in its ability to produce a distinct, measurable color change, making it an essential tool for quantitative assays and diagnostic tests. This compound is critically needed by manufacturers and research laboratories in the life sciences, pharmaceutical development, and clinical diagnostics sectors.

Application

• Cell Viability and Proliferation Assays (e.g., MTT Assay Alternative): Used as a substrate in colorimetric assays to measure metabolic activity in live cells.
• Enzyme-Linked Immunosorbent Assay (ELISA): Serves as a chromogenic substrate for detecting and quantifying specific proteins, antibodies, or antigens.
• Microbiology and Antimicrobial Susceptibility Testing: Employed to assess bacterial growth and the efficacy of antimicrobial agents.
• Biochemical Research Reagent: Acts as an electron acceptor in studies of dehydrogenase and reductase enzyme activity.
• Diagnostic Kit Manufacturing: A key component in the formulation of commercial test kits for clinical and research use.
• High-Throughput Screening (HTS): Utilized in automated screening platforms within drug discovery to identify bioactive compounds.

Basic Information

Product Name	Sodium 4-[3-(4-Iodophenyl)-2-(2,4-Dinitrophenyl)-2H-5-Tetrazolio]-1,3-Benzene Disulfonate
CAS No.	161617-45-4
Molecular Formula	C19H10IN6Na2O10S2
Molecular Weight	810.33 g/mol
Synonyms	WST-1; Water Soluble Tetrazolium Salt 1; Sodium 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, sodium salt; 4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzenedisulfonate, disodium salt; Tetrazolium Salt WST-1; Cell Counting Kit-8 (CCK-8) Reagent Component; Disodium 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonatophenyl)-2H-tetrazol-3-ium
EINECS	Contact for details

Quality Control

Our production of Sodium 4-[3-(4-Iodophenyl)-2-(2,4-Dinitrophenyl)-2H-5-Tetrazolio]-1,3-Benzene Disulfonate adheres to stringent quality management protocols to ensure batch-to-batch consistency and performance reliability for sensitive assays. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term stability, consider storage under desiccant or inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange-red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Absorbance (450 nm, Solution)	Within specified range
Heavy Metals (as Pb)	≤ 20 ppm
Biological Performance (Assay)	Meets reference standard activity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.