Description

Mt1-Mmp is a synthetic matrix metalloproteinase inhibitor with high selectivity for MT1-MMP (MMP-14), a key enzyme involved in extracellular matrix remodeling and tumor cell invasion. It serves as a critical pharmacological tool for cancer metastasis research, mechanistic enzymology studies, and preclinical target validation. Researchers in oncology drug discovery, academic biomedical laboratories, and contract research organizations (CROs) rely on Mt1-Mmp CAS NO 161384-17-4 for reproducible, high-fidelity inhibition assays under controlled experimental conditions.

Application

• Oncology research: Inhibition of MT1-MMP–mediated collagen degradation in 3D tumor spheroid and organoid invasion models
• Drug discovery screening: Primary and secondary assay standard for MMP-14–targeted compound evaluation
• Cell migration and invasion studies: Quantitative assessment of pericellular proteolysis in endothelial and carcinoma cell lines
• Biomarker validation: Functional correlation of MT1-MMP activity with cleavage fragments (e.g., CD44, LRP-1)
• Structural biology: Co-crystallization ligand for X-ray crystallographic analysis of MMP-14 catalytic domain
• Pharmacodynamic modeling: Reference compound for PK/PD relationship development in xenograft efficacy studies

Basic Information

Product Name	Mt1-Mmp
CAS No.	161384-17-4
Molecular Formula	C₂₄H₂₈N₆O₃S
Molecular Weight	480.59 g/mol
Synonyms	NSC 742521; NSC-742521; N-[4-(Hydroxycarbamoyl)phenyl]-N'-[2-(4-methylpiperazin-1-yl)pyrimidin-5-yl]thiourea; MMP-14 inhibitor I; MT1-MMP inhibitor I; Compound 1 (J. Med. Chem. 2005); 5-(4-Methylpiperazin-1-yl)-N-[4-(hydroxycarbamoyl)phenyl]pyrimidine-2-carbothioamide; NSC742521
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch of Mt1-Mmp is verified for identity (HPLC retention time, IR spectroscopy), assay purity (≥98.5% by HPLC), residual solvents (GC), heavy metals (<5 ppm), and water content (Karl Fischer). Certificates of Analysis (COA) are available upon request and conform to ICH Q5, Q7, and USP General Chapters <1058> Analytical Instrument Qualification.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8°C under inert atmosphere (argon or nitrogen) to prevent oxidation and moisture uptake. Keep desiccated with silica gel in the original sealed packaging. Avoid repeated exposure to ambient humidity and room temperature.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.5%
Related Substances (HPLC)	Single impurity ≤0.5%; Total impurities ≤1.5%
Residual Solvents (GC)	Meets ICH Q3C limits
Water (KF)	≤0.5%
Heavy Metals	<5 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.