Description

n-[2-(Dansylamino)Ethyl]Maleimide is a specialized heterobifunctional crosslinking reagent that combines a maleimide group with a dansyl fluorophore via an ethylenediamine linker. This compound matters for its dual functionality, enabling the covalent conjugation of thiol-containing biomolecules while providing a built-in fluorescent probe for detection and quantification. It is primarily needed by researchers and manufacturers in bioconjugation, proteomics, and diagnostic assay development, where precise labeling and tracking of proteins, peptides, and other biomolecules are critical.

Application

• Protein and Antibody Labeling: Covalent attachment of fluorescent tags to cysteine residues for imaging, flow cytometry, and Western blot analysis.
• Bioconjugate Synthesis: Creation of stable thioether-linked conjugates for drug delivery systems, antibody-drug conjugates (ADCs), and biosensors.
• Proteomics Research: Site-specific modification of peptides and proteins to study structure, function, and protein-protein interactions.
• Diagnostic Assay Development: Fluorescent labeling of probes and capture molecules in ELISA, lateral flow assays, and other diagnostic platforms.
• Fluorescence Quenching Studies: Utilization as a donor or acceptor in Förster resonance energy transfer (FRET) based assays.
• Polymer and Material Science: Functionalization of thiolated surfaces, nanoparticles, or polymers for advanced material applications.

Basic Information

Product Name	n-[2-(Dansylamino)Ethyl]Maleimide
CAS No.	160291-45-2
Molecular Formula	C19H21N3O4S
Molecular Weight	387.45 g/mol
Synonyms	N-(2-((5-Dimethylaminonaphthalene-1-sulfonyl)amino)ethyl)maleimide; Dansyl ethylenediamine maleimide; DED-Maleimide; 1-[2-[[5-(Dimethylamino)-1-naphthalenyl]sulfonyl]amino]ethyl]-1H-pyrrole-2,5-dione; Maleimide, N-[2-[(5-dimethylamino-1-naphthalenesulfonyl)amino]ethyl]-; DansylEDA-maleimide; N-(2-((5-(Dimethylamino)naphthalene-1-sulfonamido)ethyl)maleimide
EINECS	Contact for details

Quality Control

Our n-[2-(Dansylamino)Ethyl]Maleimide is manufactured under strict quality protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for research and development. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term storage, consider desiccation and under an inert atmosphere to maintain optimal stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange powder or crystalline solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.