Description

H-D-Phe-Pip-Arg-Pna Hydrochloride is a high-purity, synthetic peptide derivative used as a critical chromogenic substrate in biochemical research and diagnostic applications. Its core value lies in its specificity for serine proteases, enabling precise enzymatic activity measurement and inhibitor screening. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and in-vitro diagnostic (IVD) sectors who require reliable and consistent substrates for assay development and quality control.

Application

• Chromogenic Substrate for Serine Proteases: Primary application for measuring the activity of enzymes like thrombin, trypsin, and related proteases in kinetic assays.
• Biochemical Research & Drug Discovery: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential therapeutic agents.
• In-Vitro Diagnostic (IVD) Kits: A key component in diagnostic test kits for coagulation disorders, liver function, and other clinical assays requiring precise protease activity quantification.
• Enzyme Characterization: Employed to determine kinetic parameters (Km, Vmax) and study the mechanism of action of target proteases.
• Quality Control in Biopharmaceuticals: Used to monitor and validate the activity and purity of enzyme-based biopharmaceutical products during manufacturing.

Basic Information

Product Name	H-D-Phe-Pip-Arg-Pna Hydrochloride
CAS No.	160192-34-7
Molecular Formula	C30H44ClN9O5
Molecular Weight	646.19 g/mol
Synonyms	D-Phenylalanyl-piperidyl-arginyl-p-nitroanilide hydrochloride; H-D-Phe-Pip-Arg-pNA · HCl; Chromogenic Substrate for Thrombin; Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride (related analog); Bz-Phe-Val-Arg-pNA · HCl (common trade name); Thrombin Substrate I; Tosyl-Gly-Pro-Arg-p-nitroanilide acetate (alternative sequence); Protease Substrate pNA; p-Nitroanilide (pNA) Peptide Substrate.
EINECS	Contact for details

Quality Control

Our H-D-Phe-Pip-Arg-Pna Hydrochloride is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and rigorous impurity profiling. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term stability, store desiccated under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.