Description

Vitamin B12 E-Nhs Ester is a chemically activated derivative of cyanocobalamin, designed for efficient covalent conjugation to primary amines in aqueous buffers. This compound is critical for researchers and manufacturers requiring stable, site-specific labeling of biomolecules. It is primarily utilized in the development of diagnostic assays, bioconjugation for targeted drug delivery, and advanced biochemical research.

Application

• Bioconjugation & Protein Labeling: Covalently attaches Vitamin B12 to antibodies, peptides, or other proteins for creating targeted imaging and therapeutic agents.
• Diagnostic Assay Development: Used as a key reagent in the synthesis of enzyme-linked immunosorbent assay (ELISA) components and other immunoassay tracers.
• Pharmaceutical Research: Serves as an intermediate in the synthesis of novel B12-drug conjugates for exploring targeted cancer therapies and nutrient delivery systems.
• Biochemical Probe Synthesis: Enables the preparation of fluorescent or affinity-tagged Vitamin B12 derivatives for studying cellular uptake and receptor binding.
• Material Science Functionalization: Used to modify surfaces (e.g., nanoparticles, polymers) with Vitamin B12 for biosensing or bioactive material applications.

Basic Information

Product Name	Vitamin B12 E-Nhs Ester
CAS No.	160158-29-2
Molecular Formula	C₆₃H₈₈CoN₁₄O₁₇P
Molecular Weight	1403.3 g/mol
Synonyms	Cyanocobalamin N-hydroxysuccinimide ester; Vitamin B12 NHS ester; Cobalamin N-succinimidyl ester; B12-NHS; N-Hydroxysuccinimide ester of Vitamin B12; Cyanocobalamin active ester; Coenzyme B12 NHS ester; 5'-Deoxyadenosylcobalamin NHS ester (related form)
EINECS	Contact for details

Quality Control

Our Vitamin B12 E-Nhs Ester is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. For custom grades or specific purity requirements, please contact our technical team.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Red to dark red powder or crystalline solid
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Research Grade)
Water Content (Karl Fischer)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.