Description

6-Chloro-3-Indolyl Phosphate p-Toluidine Salt is a specialized chromogenic substrate salt widely used in molecular biology and diagnostic applications. This compound is critical for the sensitive and specific detection of alkaline phosphatase activity in techniques such as Western blotting, ELISA, and immunohistochemistry. It is an essential reagent for research institutions, diagnostic kit manufacturers, and pharmaceutical development laboratories requiring high-purity biochemicals for precise analytical results.

Application

• Chromogenic Substrate for Alkaline Phosphatase (AP): Primary use as a precipitating substrate in Western blotting, dot/slot blotting, and membrane-based immunoassays.
• Enzyme-Linked Immunosorbent Assay (ELISA): Used in colorimetric ELISA kits for the detection of antigens or antibodies, producing an insoluble blue precipitate.
• Immunohistochemistry (IHC) & In Situ Hybridization (ISH): Enables precise localization of target proteins or nucleic acids in tissue sections.
• Diagnostic Kit Manufacturing: A key raw material in the production of clinical diagnostic kits for various diseases.
• Nucleic Acid Detection: Employed in non-radioactive labeling and detection systems for DNA/RNA probes.
• Research & Development: Essential for academic and industrial R&D in biochemistry, cell biology, and pathology.
• Quality Control (QC) Reagent: Used as a reference standard or active component in QC testing for diagnostic products.

Basic Information

Item	Details
Product Name	6-Chloro-3-Indolyl Phosphate p-Toluidine Salt
CAS No.	159954-33-3
Molecular Formula	C21H20ClN2O4P
Molecular Weight	430.82 g/mol
Synonyms	BCIP p-Toluidine Salt; 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (common related name); 6-Chloro-3-indolyl phosphate toluidine salt; 6-Cl-IP p-Toluidine Salt; 6-Chloroindoxyl phosphate p-toluidine salt; AP Chromogenic Substrate; Alkaline Phosphatase Substrate, BCIP analog; 3-Indolyl phosphate, 6-chloro-, p-toluidine salt
EINECS	Contact for details

Quality Control

Our 6-Chloro-3-Indolyl Phosphate p-Toluidine Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and spectroscopic identification, to ensure it meets the high standards required for sensitive diagnostic and research applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.