Description

Transcription Factor Tfiiir CAS NO 158415-69-1 is a highly purified, biologically active protein complex essential for the initiation of RNA polymerase III-mediated transcription. This product is critical for researchers and developers in the life sciences sector who require reliable, high-quality reagents for studying eukaryotic gene expression mechanisms. It is primarily utilized in biochemical research, molecular biology assays, and the development of novel therapeutic screening platforms.

Application

• Biochemical Research: Used in in vitro transcription assays to study the regulation and assembly of the RNA polymerase III pre-initiation complex.
• Molecular Biology Studies: Essential for investigating promoter recognition, transcription initiation, and the role of specific transcription factors in gene expression.
• Drug Discovery & Screening: Serves as a key component in high-throughput screening platforms to identify potential modulators of RNA polymerase III activity for therapeutic development.
• Enzymology: Employed in kinetic and mechanistic studies to understand the catalytic cycle and co-factor requirements of the transcription machinery.
• Diagnostic Development: Potential use as a reference standard or critical reagent in the development of assays targeting transcription-related disorders.

Basic Information

Product Name	Transcription Factor Tfiiir
CAS No.	158415-69-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	TFIIIR; Transcription Factor III R; RNA Polymerase III Transcription Factor R; General Transcription Factor 3R; GTF3R; TFIII R Complex
EINECS	Contact for details

Quality Control

Our Transcription Factor Tfiiir is produced under stringent conditions to ensure batch-to-batch consistency and biological activity. Each lot undergoes rigorous quality testing, including purity assessment via SDS-PAGE and functional activity validation in standardized transcription assays. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, activity, and storage buffer composition is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive) and light-sensitive; handle under appropriate conditions to maintain activity.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Identification (SDS-PAGE)	Corresponds to reference pattern
Purity (SDS-PAGE)	> 90%
Protein Concentration	Provided on COA
Biological Activity	Functional in in vitro transcription assay
Endotoxin Level	< 1.0 EU/µg
Buffer	20 mM HEPES, pH 7.9, 100 mM KCl, 0.2 mM EDTA, 20% Glycerol, 1 mM DTT

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.