Description

Sulfo-n-Succinimidyl 4-Maleimido- CAS NO 158018-81-6 is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysulfosuccinimide (sulfo-NHS) ester and a thiol-reactive maleimide group. This compound is essential for creating stable, site-specific conjugates between proteins, peptides, and other biomolecules, enabling advanced research and diagnostic applications. It is a critical tool for scientists and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and immunoassay production.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the controlled attachment of cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Protein-Protein/Peptide Conjugation: Enables the stable coupling of different proteins or peptides to create novel bifunctional reagents or fusion protein mimics.
• Enzyme and Fluorescent Probe Labeling: Used to attach enzymes (e.g., HRP) or fluorescent dyes to antibodies or other targeting molecules for ELISA and other immunoassays.
• Surface Immobilization: Facilitates the covalent attachment of biomolecules to sensor chips, beads, or other solid supports for biosensor and microarray development.
• Nanoparticle Functionalization: Aids in decorating nanoparticles with targeting ligands or therapeutic agents for drug delivery systems.
• Diagnostic Reagent Manufacturing: Key component in the synthesis of stable, high-performance reagents used in clinical diagnostics.

Basic Information

Product Name	Sulfo-n-Succinimidyl 4-Maleimido- CAS NO 158018-81-6
CAS No.	158018-81-6
Molecular Formula	C14H11N2O10S2- • Na+
Molecular Weight	454.36 g/mol
Synonyms	Sulfo-SMCC; 4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt; Sodium 4-[(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)methyl]cyclohexane-1-carboxylate 2,5-dioxo-1-(sulfooxy)pyrrolidin-3-yl ester; SMCC Sulfo-NHS ester; Sulfo-N-succinimidyl 4-(maleimidomethyl)cyclohexanecarboxylate; Heterobifunctional crosslinker sulfo-SMCC; NHS-PEG4-Maleimide (sulfo analog); Water-soluble SMCC crosslinker.
EINECS	Contact for details

Quality Control

Our Sulfo-n-Succinimidyl 4-Maleimido- CAS NO 158018-81-6 is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive bioconjugation applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and functional performance is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO
Functional Test	Conjugation efficiency confirmed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.