Description

(Tyr65,Phe67)-C5A (65-74) (Human) is a synthetic peptide fragment corresponding to a specific region of the human C5a anaphylatoxin protein with defined amino acid substitutions. This modified sequence is a critical research tool for studying the structure-activity relationships and receptor interactions of the complement system component C5a. It is primarily utilized by researchers in immunology, inflammation, and drug discovery to investigate signaling pathways and develop novel therapeutic inhibitors.

Application

• Biochemical Research: Used as a reference standard or active ligand in studies of the complement C5a signaling cascade and its role in inflammatory diseases.
• Receptor Binding Assays: Essential for competitive binding studies to characterize the affinity and specificity of the C5a receptor (C5aR1, CD88).
• Drug Discovery & Screening: Serves as a key reagent in high-throughput screening (HTS) campaigns to identify and validate potential C5a receptor antagonists.
• Antibody Development: Can be used as an immunogen or for epitope mapping in the development of monoclonal antibodies targeting the C5a protein.
• Inflammation Modeling: Applied in in vitro cellular assays to induce and study inflammatory responses mediated by C5a receptor activation.
• Structure-Activity Relationship (SAR) Studies: The specific Tyr and Phe substitutions make it invaluable for probing the importance of these residues for biological activity.

Basic Information

Product Name	(Tyr65,Phe67)-C5A (65-74) (Human)
CAS No.	157952-15-3
Molecular Formula	C58H81N13O14
Molecular Weight	1184.35 g/mol
Synonyms	C5a (65-74) (human) Tyr65,Phe67 analog; Complement C5a anaphylatoxin (65-74) fragment, [Tyr65,Phe67]-; [Tyr65, Phe67]-C5a (65-74); H-Tyr-Met-Val-Met-Leu-Phe-Leu-Ala-Arg-OH (Tyr65, Phe67 variant); C5a receptor ligand analog; CAS 157952-15-3; Human complement C5a decapeptide (65-74) analog
EINECS	Contact for details

Quality Control

Our (Tyr65,Phe67)-C5A (65-74) (Human) is produced under stringent conditions to ensure batch-to-batch consistency and high purity suitable for research applications. Each lot is supported by comprehensive analytical data. Certificates of Analysis (COA) are provided, detailing results for purity (by HPLC), peptide content, amino acid analysis, and mass spectrometry (MS) confirmation.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, keep under inert atmosphere or in a vacuum desiccator. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Amino Acid Analysis	Within ± 10% of theoretical
Single Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.