Description

Collagen Type IV Alpha3 Chain (185-203) is a defined synthetic peptide fragment corresponding to a specific sequence within the alpha3 chain of Type IV collagen. This high-purity peptide is critical for advanced biomedical research, particularly in studies of basement membrane biology and associated pathologies. It serves as an essential tool for researchers and developers in the fields of immunology, nephrology, and targeted therapeutic discovery, enabling precise investigation of molecular interactions and autoimmune epitopes.

Application

• Biomedical Research: Used as a key antigen in immunological assays to study autoimmune diseases like Goodpasture syndrome, where antibodies target this specific collagen epitope.
• Epitope Mapping: Essential for defining and characterizing antibody-binding sites (epitopes) on the Type IV collagen alpha3 chain.
• Diagnostic Development: Serves as a coating antigen or standard in ELISA and other immunoassays for detecting and quantifying specific autoantibodies in patient sera.
• Therapeutic Target Investigation: Facilitates research into novel therapeutic strategies, including tolerization and blocking antibody therapies, for autoimmune kidney and lung diseases.
• Structural Biology Studies: Employed in biophysical and structural studies to understand the molecular conformation and interactions of the collagen IV network.
• Vaccine Research: Investigated in preclinical studies for developing antigen-specific immunotherapies.
• Quality Control Reagent: Acts as a reference standard for the quality control of diagnostic kits targeting anti-collagen IV antibodies.

Basic Information

Item	Detail
Product Name	Collagen Type IV Alpha3 Chain (185-203)
CAS No.	157802-35-2
Molecular Formula	C92H147N29O30
Molecular Weight	2095.3 g/mol
Synonyms	Collagen α-3(IV) Chain (185-203); Goodpasture Antigen Peptide; Alpha3(IV)NC1 Peptide; Human Collagen Type IV Alpha3 Chain Peptide (185-203); GP Peptide; COL4A3 (185-203); NC1 Domain Peptide; Autoantigen Peptide
EINECS	Contact for details

Quality Control

Every batch of Collagen Type IV Alpha3 Chain (185-203) is manufactured and analyzed under strict quality management systems. Our products undergo rigorous purity and identity verification using advanced analytical techniques including HPLC and mass spectrometry to ensure >95% purity (by HPLC) and correct sequence confirmation. Certificates of Analysis (COA) detailing batch-specific results are provided and available upon request to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Retention time and mass correspond to reference
Purity (HPLC)	≥ 95%
Amino Acid Sequence	H-Gly-Val-Lys-Gly-Asp-Lys-Gly-Asn-Pro-Gly-Trp-Pro-Gly-Ala-Pro-Gly-Pro-Lys-NH2
Molecular Weight	2095.3 ± 1.0 Da
Peptide Content	≥ 70% (by weight)
Solubility	Soluble in water or dilute acetic acid

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.