Description

2,3-Di-(4-Nitrophenyl)-5-T-Butyl-2H-Tetrazolium is a specialized tetrazolium salt derivative, primarily recognized for its role as a critical reagent in biochemical assays. Its core value lies in its ability to serve as a substrate for dehydrogenase enzymes, producing a measurable formazan dye that is essential for cell viability and cytotoxicity testing. This compound is therefore indispensable for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, where precise quantification of metabolic activity is required.

Application

• Cell Viability and Proliferation Assays (e.g., MTT, XTT): Acts as a key substrate in colorimetric assays to measure mitochondrial activity in live cells.
• Cytotoxicity Screening: Used in high-throughput screening of pharmaceutical compounds and chemicals for toxic effects on cultured cells.
• Microbiology Research: Employed in studies of microbial dehydrogenase activity and metabolic function.
• Biochemical Research Reagent: Serves as an electron acceptor in enzymatic studies involving NAD(P)H-dependent oxidoreductases.
• Diagnostic Kit Manufacturing: A component in the formulation of diagnostic kits for clinical and research applications.
• Material Science: Investigated for potential use in redox-active materials and sensors due to its distinct electrochemical properties.

Basic Information

Product Name	2,3-Di-(4-Nitrophenyl)-5-T-Butyl-2H-Tetrazolium
CAS No.	157458-76-9
Molecular Formula	C23H20N6O4
Molecular Weight	444.45 g/mol
Synonyms	5-tert-Butyl-2,3-bis(4-nitrophenyl)-2H-tetrazolium; 5-tert-Butyl-2,3-di(4-nitrophenyl)tetrazolium; 2,3-Bis(4-nitrophenyl)-5-tert-butyl-2H-tetrazolium; 2,3-Di(4-nitrophenyl)-5-tert-butyltetrazolium; TNBT; Tetranitro Blue Tetrazolium (common name); 2H-Tetrazolium, 2,3-bis(4-nitrophenyl)-5-(1,1-dimethylethyl)-
EINECS	Contact for details

Quality Control

Our 2,3-Di-(4-Nitrophenyl)-5-T-Butyl-2H-Tetrazolium is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption, which can affect stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.