Description

7-Diethylamino-3-[n-(2-Maleimidoethyl)Carbamoyl]Coumarin is a specialized, bifunctional fluorescent probe that combines a bright coumarin fluorophore with a reactive maleimide group. This compound matters for researchers and developers requiring site-specific labeling of thiol groups in biomolecules with a sensitive, environmentally-sensitive fluorescent tag. It is primarily needed by professionals in bioconjugation, proteomics, and bioassay development for creating advanced diagnostic tools and studying protein interactions.

Application

• Site-Specific Protein Labeling: Covalent attachment to cysteine residues via the maleimide group for fluorescence tagging.
• Bioconjugate Synthesis: Creation of fluorescent antibody-drug conjugates (ADCs) or peptide probes for targeted imaging.
• Fluorescence Sensing: Development of "turn-on" or rationetric sensors where the coumarin fluorescence is modulated by the local environment.
• Biomolecular Interaction Studies: Use in fluorescence polarization (FP) or Förster resonance energy transfer (FRET) assays to monitor binding events.
• Diagnostic Reagent Formulation: A key component in the synthesis of reagents for clinical diagnostics and high-throughput screening (HTS).
• Polymer and Material Science: Functionalization of thiol-containing polymers or surfaces for creating smart, fluorescent materials.

Basic Information

Item	Details
Product Name	7-Diethylamino-3-[n-(2-Maleimidoethyl)Carbamoyl]Coumarin
CAS No.	156571-46-9
Molecular Formula	C21H23N3O5
Molecular Weight	397.43 g/mol
Synonyms	7-Diethylaminocoumarin-3-carboxylic acid (2-maleimidoethyl)amide; Maleimide-C1-Coumarin; 3-[(2-Maleimidoethyl)carbamoyl]-7-(diethylamino)coumarin; 7-Diethylamino-3-carboxycoumarin maleimide; N-(2-Maleimidoethyl)-7-diethylaminocoumarin-3-carboxamide; Thiol-reactive coumarin 343 derivative; Coumarin 343 maleimide
EINECS	Contact for details

Quality Control

Our production of this high-value biochemical is governed by stringent quality protocols to ensure batch-to-batch consistency and performance reliability. Every lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and the integrity of the reactive maleimide group. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	~430-450 nm
Emission (λmax)	~470-490 nm
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.