Description

Rad23 Protein, Human is a highly purified recombinant protein crucial for nucleotide excision repair (NER) and other DNA damage response pathways. This protein serves as an essential research tool for studying the molecular mechanisms of DNA repair, genomic stability, and associated diseases like cancer. It is primarily utilized by academic, pharmaceutical, and biotechnology researchers focused on molecular biology, biochemistry, and oncology.

Application

• Biochemical Research: Used as a key reagent in in vitro assays to study protein-protein interactions within the DNA damage recognition complex.
• Mechanistic Studies: Essential for investigating the role of Rad23 in ubiquitin-mediated proteolysis and its shuttle function in NER.
• Drug Discovery Screening: Employed in high-throughput screening platforms to identify potential therapeutic compounds that modulate DNA repair pathways.
• Protein Interaction Mapping: Serves as bait or prey in pull-down assays, co-immunoprecipitation, and yeast two-hybrid screens to map interactomes.
• Enzyme Activity Assays: Used in reconstituted systems to study the activation and regulation of repair-associated enzymatic activities.
• Antibody Production & Validation: Acts as a high-purity antigen for generating and validating specific polyclonal or monoclonal antibodies.

Basic Information

Product Name	Rad23 Protein, Human
CAS No.	156533-33-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	RAD23A; HR23A; hHR23A; UV excision repair protein RAD23 homolog A; XP-C repair-complementing complex 58 kDa protein; HHR23A; Human homolog of yeast Rad23; DNA repair protein RAD23 homolog A
EINECS	Contact for details

Quality Control

Our Rad23 Protein, Human is produced under stringent conditions and undergoes rigorous analytical testing to ensure high purity, identity, and functional activity. Each batch is validated using techniques including SDS-PAGE, Western Blot, and functional binding assays. Certificates of Analysis (COA) detailing purity, concentration, and specific activity are provided to guarantee consistency and reliability for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	≥ 95%
Identification (Western Blot)	Positive
Protein Concentration	Provided on COA
Buffer Composition	20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH 8.0
Endotoxin Level	< 1.0 EU/µg
Biological Activity	Confirmed by functional binding assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.