Description

Cytochrome p-450 Ef CAS NO 156320-24-0 is a highly purified enzyme preparation belonging to the cytochrome P450 superfamily, which plays a critical role in the oxidative metabolism of a vast array of endogenous and exogenous compounds. This product is essential for researchers and developers in life sciences, enabling critical studies in drug metabolism, toxicology, and biochemical pathway analysis. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions focused on enzymology and metabolic studies.

Application

• Drug Metabolism and Interaction Studies: Used as a standard enzyme source for in vitro assays to predict metabolic stability, identify metabolites, and assess drug-drug interaction potential.
• Enzyme Kinetics and Characterization: Employed in fundamental research to study catalytic mechanisms, inhibition constants (Ki), and enzyme induction properties of specific P450 isoforms.
• Toxicology and Safety Assessment: Integral for evaluating the bioactivation of protoxins and procarcinogens, aiding in the understanding of compound toxicity pathways.
• Biocatalysis and Synthesis: Applied in the development of enzymatic synthesis routes for chiral intermediates and specialty chemicals due to its regio- and stereoselective oxidation capabilities.
• Diagnostic Kit Development: Serves as a key component in the manufacturing of diagnostic kits and reagents designed to measure specific metabolic functions.
• Educational and Reference Material: Used in advanced biochemistry and pharmacology teaching laboratories as a high-quality reference standard for practical experiments.

Basic Information

Product Name	Cytochrome p-450 Ef
CAS No.	156320-24-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cytochrome P450 Ef; CYP450 Ef; P450 Ef; Heme-thiolate monooxygenase Ef; Microsomal cytochrome P450 Ef; Xenobiotic monooxygenase Ef; Steroid 11β-monooxygenase; [Cytochrome P-450] Ef
EINECS	Contact for details

Quality Control

Our Cytochrome p-450 Ef is produced under stringent quality management protocols to ensure batch-to-batch consistency and high biological activity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as specific activity, purity profile, and absence of contaminating activities. We adhere to relevant quality guidelines suitable for research and development applications to guarantee reliable and reproducible performance in your assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use to minimize exposure to atmospheric moisture. For short-term use, thaw on ice and keep aliquots cold. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (IR/Spectra)	Conforms to standard
Specific Activity	≥ Contact for details (nmol/min/mg protein)
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	Contact for details
Buffer Composition	Contact for details
Stability	Stable for specified period when stored as directed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.