Description

Nedd1 Protein CAS NO 155982-08-4 is a highly purified recombinant protein, also known as Neural precursor cell expressed developmentally down-regulated protein 1, which plays a critical role in the ubiquitin-proteasome pathway. This protein is essential for fundamental biochemical research into protein degradation, cell cycle regulation, and signal transduction mechanisms. It is primarily utilized by researchers and developers in the fields of molecular biology, oncology, and neurodegenerative disease research, where understanding protein regulation is paramount.

Application

• Biochemical Research: Used as a key reagent in in vitro studies to investigate the NEDDylation pathway and its role in protein stability and function.
• Drug Discovery & Target Validation: Serves as a critical target or tool compound in high-throughput screening assays for developing novel therapeutics targeting ubiquitin-like pathways.
• Enzymology Studies: Employed in enzymatic activity assays to study the kinetics and mechanisms of NEDD8-activating enzyme E1.
• Protein-Protein Interaction Analysis: Used in pull-down assays, co-immunoprecipitation, and surface plasmon resonance (SPR) to map interaction networks.
• Cell Signaling Pathway Analysis: A vital component in research focused on understanding cellular responses to stress, DNA damage, and oncogenic signals.
• Antibody Production & Validation: Acts as a high-purity antigen for the generation and specificity testing of monoclonal and polyclonal antibodies.

Basic Information

Product Name	Nedd1 Protein
CAS No.	155982-08-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Neural precursor cell expressed developmentally down-regulated protein 1; NEDD1; NEDD-1; Nedd1 Protein; NEDD8-activating enzyme E1 catalytic subunit; Ubiquitin-like modifier NEDD8-activating enzyme E1; NAE1; APP-BP1; UBA3-APPBP1 complex; DKFZp686O24128
EINECS	Contact for details

Quality Control

Our Nedd1 Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and HPLC, functional activity assays, and endotoxin level verification. Certificates of Analysis (COA) detailing all specifications are available upon request to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. Aliquot to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Purity (SDS-PAGE)	≥ 95%
Identification (Mass Spec)	Conforms
Biological Activity	Assay-dependent; COA provided
Endotoxin Level	< 1.0 EU/µg
Protein Concentration	As per COA
Buffer Composition	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.